The present application provides a method for lysis or electroporation of cells in a biological sample including the following steps: passing cells of the sample, suspended in a fluid, through a flow path with a preset flow speed, wherein the flow path runs through a detection apparatus for detecting individual cells and wherein the flow path includes at least two electrodes for generating an electric field, which electrodes are located downstream of the detection apparatus and which electrodes are coated with a dielectric material with a relative permittivity greater than 3.9, wherein the coaling at least covers the surface of the electrodes that faces the flow path, and when the presence of a specific cell is detected in the detection apparatus, then an electric field is generated between the electrodes when the detected cell passes between the electrodes in dependence of the flow speed, wherein the electric field causes electroporation or lysis of the cell.

The present application provides a method for lysis or electroporation of cells in a biological sample including the following steps: passing cells of the sample, suspended in a fluid, through a flow path with a preset flow speed, wherein the flow path runs through a detection apparatus for detecting individual cells and wherein the flow path includes at least two electrodes for generating an electric field, which electrodes are located downstream of the detection apparatus and which electrodes are coated with a dielectric material with a relative permittivity greater than 3.9, wherein the coaling at least covers the surface of the electrodes that faces the flow path, and when the presence of a specific cell is detected in the detection apparatus, then an electric field is generated between the electrodes when the detected cell passes between the electrodes in dependence of the flow speed, wherein the electric field causes electroporation or lysis of the cell.

A method of depositing single particles onto a target comprises the steps of loading a particle suspension to a droplet dispenser having a suspension reservoir and a nozzle section, detecting particles in the nozzle section, testing a single particle condition of the droplet dispenser, wherein it is determined whether an ejection region of the nozzle section includes one single particle, and operating the droplet dispenser for dispensing a droplet, wherein the droplet is dispensed onto the target, if the single particle condition is fulfilled, or the droplet is dispensed into a collection reservoir, if the single particle condition is not fulfilled, wherein the step of testing the single particle condition further includes determining whether a sedimentation region adjacent to the ejection region is free of particles. Furthermore, a dispenser apparatus dispensing single particles onto a target is described.

A method of depositing single particles onto a target comprises the steps of loading a particle suspension to a droplet dispenser having a suspension reservoir and a nozzle section, detecting particles in the nozzle section, testing a single particle condition of the droplet dispenser, wherein it is determined whether an ejection region of the nozzle section includes one single particle, and operating the droplet dispenser for dispensing a droplet, wherein the droplet is dispensed onto the target, if the single particle condition is fulfilled, or the droplet is dispensed into a collection reservoir, if the single particle condition is not fulfilled, wherein the step of testing the single particle condition further includes determining whether a sedimentation region adjacent to the ejection region is free of particles. Furthermore, a dispenser apparatus dispensing single particles onto a target is described.

The present application generally pertains to scaling of a production process to produce a chemical, pharmaceutical and/or biotechnological product and/or of a production state of a respective production equipment. Particularly, there is provided a computer-implemented method of scaling a production process to produce a chemical, pharmaceutical and/or biotechnological product, the scaling being from a source scale to a target scale, wherein the production process is defined by a plurality of steps specified by one or more process parameters controlling an execution of the production process, the method comprising: (a) retrieving: parameter evolution information that describes the time evolution of the process parameter(s); a plurality of recipe templates, wherein a recipe comprises the plurality of steps defining the production process, and wherein a recipe template is a recipe in which at least one of the process parameters specifying the plurality of steps is a parameter being variable and having no predetermined value at the outset; (b) receiving: a source setup specification of a source setup to be used for executing the production process at the source scale, the source setup specification comprising the source scale value: a target setup specification of a target setup to be used for executing the production process at the target scale, the target setup specification comprising the target scale value; a source recipe defining the production process at the source scale: at least one acceptability function defining conditions for the values of the process parameter(s) at the source scale and/or at the target scale; (c) simulating the execution of the production process at the source scale using the source setup specification, the source recipe and the parameter evolution information: (d) determining, from the simulation, one or more source trajectories for the process parameters), wherein a trajectory corresponds to a time-based profile of values recordable during the simulated execution of the production process; (e) performing a target determination step comprising: selecting a recipe template pertinent to the production process out of the plurality of recipe templates; providing an input value for the at least one variable parameter in the selected recipe template; simulating the execution of the production process at the target scale using the target setup specification, the selected recipe template, the input value for the at least one variable parameter and the parameter evolution information; determining, from the simulation, one or more target trajectories for the process parameters; comparing the source trajectory(ies) and the target trajector

The present application generally pertains to scaling of a production process to produce a chemical, pharmaceutical and/or biotechnological product and/or of a production state of a respective production equipment. Particularly, there is provided a computer-implemented method of scaling a production process to produce a chemical, pharmaceutical and/or biotechnological product, the scaling being from a source scale to a target scale, wherein the production process is defined by a plurality of steps specified by one or more process parameters controlling an execution of the production process, the method comprising: (a) retrieving: parameter evolution information that describes the time evolution of the process parameter(s); a plurality of recipe templates, wherein a recipe comprises the plurality of steps defining the production process, and wherein a recipe template is a recipe in which at least one of the process parameters specifying the plurality of steps is a parameter being variable and having no predetermined value at the outset; (b) receiving: a source setup specification of a source setup to be used for executing the production process at the source scale, the source setup specification comprising the source scale value: a target setup specification of a target setup to be used for executing the production process at the target scale, the target setup specification comprising the target scale value; a source recipe defining the production process at the source scale: at least one acceptability function defining conditions for the values of the process parameter(s) at the source scale and/or at the target scale; (c) simulating the execution of the production process at the source scale using the source setup specification, the source recipe and the parameter evolution information: (d) determining, from the simulation, one or more source trajectories for the process parameters), wherein a trajectory corresponds to a time-based profile of values recordable during the simulated execution of the production process; (e) performing a target determination step comprising: selecting a recipe template pertinent to the production process out of the plurality of recipe templates; providing an input value for the at least one variable parameter in the selected recipe template; simulating the execution of the production process at the target scale using the target setup specification, the selected recipe template, the input value for the at least one variable parameter and the parameter evolution information; determining, from the simulation, one or more target trajectories for the process parameters; comparing the source trajectory(ies) and the target trajector

Provided is a method for facilitating an aerobic fermentation reaction using combustion waste gas, wherein organic waste in a reactor is heated using waste gas with heat produced from fuel combustion to facilitate the fermentation reaction, some of the chemical substances in the waste gas with heat produced from fuel combustion are absorbed by the organic waste in the reactor, and an environmental friendly treatment is performed on the waste gas with heat produced from fuel combustion. The specific practices comprise: connecting a gas outlet of a combustion device to a conveying pipe, and then connecting the conveying pipe to a gas inlet of the reactor or an air chamber aeration nozzle at a lower part of the reactor. Not only the equipment and cost for combustion waste gas treatments are saved, but also the aerobic fermentation efficiency of the organic waste is improved, which reduces the fermentation time. Not only the combustion waste gas is treated for environmental protection, but also the environmental friendly treatments of organic refuse waste are facilitated; and thus the method is an energy saving and environmental friendly technique.

Provided is a method for facilitating an aerobic fermentation reaction using combustion waste gas, wherein organic waste in a reactor is heated using waste gas with heat produced from fuel combustion to facilitate the fermentation reaction, some of the chemical substances in the waste gas with heat produced from fuel combustion are absorbed by the organic waste in the reactor, and an environmental friendly treatment is performed on the waste gas with heat produced from fuel combustion. The specific practices comprise: connecting a gas outlet of a combustion device to a conveying pipe, and then connecting the conveying pipe to a gas inlet of the reactor or an air chamber aeration nozzle at a lower part of the reactor. Not only the equipment and cost for combustion waste gas treatments are saved, but also the aerobic fermentation efficiency of the organic waste is improved, which reduces the fermentation time. Not only the combustion waste gas is treated for environmental protection, but also the environmental friendly treatments of organic refuse waste are facilitated; and thus the method is an energy saving and environmental friendly technique.

A method for detecting inoculation in order to detect inoculation with a sample of a solid culture medium (21) present in a layer on a culture plate (20), said culture plate (20) being positioned on a support (2), said method comprising a step of projecting an incident light flux towards said support (2) in order to allow oblique illumination of the surface referred to as the inoculation surface (22) of said culture medium (21) of the culture plate (20) when the culture plate (20) is provided in the disposed state on said support (2), a step of displaying, on a display area (41) of a display screen (4), a two-dimensional image of the incident light flux reflected by the inoculation surface (22) of the culture plate (20) in the illuminated state illuminated by the means (3) for projecting a light flux, and a step of photographing the display area (41) of the screen (4).

A method for detecting inoculation in order to detect inoculation with a sample of a solid culture medium (21) present in a layer on a culture plate (20), said culture plate (20) being positioned on a support (2), said method comprising a step of projecting an incident light flux towards said support (2) in order to allow oblique illumination of the surface referred to as the inoculation surface (22) of said culture medium (21) of the culture plate (20) when the culture plate (20) is provided in the disposed state on said support (2), a step of displaying, on a display area (41) of a display screen (4), a two-dimensional image of the incident light flux reflected by the inoculation surface (22) of the culture plate (20) in the illuminated state illuminated by the means (3) for projecting a light flux, and a step of photographing the display area (41) of the screen (4).