The present invention aims to provide a simple and efficient method for manufacturing -alumina particles, main component particles of which each have a crystal face other than the face [001] as a main crystal face and a polyhedral shape other than a hexagonal bipyramidal shape. According to the method for manufacturing -alumina particles of the present invention, when an aluminum compound is calcined in the presence of a specific content of a metal compound, -alumina particles each having a particle diameter of 50 m or less, a degree of crystallization of 90% or more, and a polyhedral shape can be obtained.

The present invention discloses a method for crystallizing recombinant Human Insulin at lab and manufacturing scale in the presence of zinc chloride and sodium chloride mixture, higher concentration of organic solvent (IPA-19 to 25 million) and adjusting the pH to 5.0 at a faster rate (5 minutes). The method further comprises adopting procedures wherein the settling time is reduced and the holding temperature is altered in order to facilitate consistent protein crystal formation between 15 m-30 m and to increase the robustness of the process.

The present invention discloses a method for crystallizing recombinant Human Insulin at lab and manufacturing scale in the presence of zinc chloride and sodium chloride mixture, higher concentration of organic solvent (IPA-19 to 25 million) and adjusting the pH to 5.0 at a faster rate (5 minutes). The method further comprises adopting procedures wherein the settling time is reduced and the holding temperature is altered in order to facilitate consistent protein crystal formation between 15 m-30 m and to increase the robustness of the process.