The invention relates to a method for selecting proteins stable in non-standard physicochemical conditions (temperature, pressure, pH, osmolarity, salinity, solvent, etc.) comprising the expression, in an extremophilic microorganism, of variants of the protein of interest in the form of a fusion protein with a reporter protein which is stable in extreme conditions and acts as a selection marker.

The present invention provides the use of RNA removal to initiate protein aggregation of a plurality of proteins in a cell or cell lysate. This may be used to create an in vitro model of a disease, such as a neurodegenerative disease. The present invention also provides a method for determining the efficacy of a potential anti-protein aggregation agent comprising the following steps: i) using RNA removal to initiate the aggregation of a protein in a cell or cell lysate, ii) treating the cell or cell lysate with the potential anti-protein aggregation agent before, after or during RNA removal; and iii) comparing protein aggregation in equivalent samples with and without step ii) treatment in which a decrease in protein aggregation associated with step ii) treatment indicates that the potential anti-protein aggregation agent is effective in preventing and/or reversing protein aggregation.

Compositions which are fragrant and contain at least a member set culled from a library of compositions, each being comprised of sub-combinations of selected terpenes. Fragrances that mimic that of various states of organic and synthetic aromatics including products, processes and those from non-combusted plant products, among other things, uniquitous products, processes, medicinals, and related moieties leverage databases of all known terpene groupings are offered for consideration, and have been provided, according to the instant teachings.

Compositions which are fragrant and contain at least a member set culled from a library of compositions, each being comprised of sub-combinations of selected terpenes. Fragrances that mimic that of various states of organic and synthetic aromatics including products, processes and those from non-combusted plant products, among other things, uniquitous products, processes, medicinals, and related moieties leverage databases of all known terpene groupings are offered for consideration, and have been provided, according to the instant teachings.

The present invention provides a method for diagnosing breast cancer comprising or consisting of the steps of (a) providing a sample to be tested; and (b) determining a biomarker signature of the test sample by measuring the presence and/or amount in the test sample of one or more biomarker selected from the group defined in Table A(i) and/or Table A(ii); wherein the presence and/or amount in the test sample of the one or more biomarker selected from the group defined in Table A(i) and/or Table A(ii) is indicative of the presence of breast cancer cells in the individual, corresponding uses, methods of treating breast cancer, together with arrays and kits for use in the same.

The present invention provides a method for diagnosing breast cancer comprising or consisting of the steps of (a) providing a sample to be tested; and (b) determining a biomarker signature of the test sample by measuring the presence and/or amount in the test sample of one or more biomarker selected from the group defined in Table A(i) and/or Table A(ii); wherein the presence and/or amount in the test sample of the one or more biomarker selected from the group defined in Table A(i) and/or Table A(ii) is indicative of the presence of breast cancer cells in the individual, corresponding uses, methods of treating breast cancer, together with arrays and kits for use in the same.

The invention provides a surface, wherein said surface comprises (i) a polymer substrate and (ii) sugar groups and peptide groups coupled to said substrate suitable for culturing hepatocytes.

A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.

A method comprising transforming Schizosaccharomyces pombe with a deletion cassette, constructed by four-round serial PCR, block PCR or total gene synthesis, containing a homologous recombination site is provided for preparing gene-targeted heterozygous deletion Schizosaccharomyces pombe. Also provided are gene-targeted hetero2ygous deletion Schizosaccharomyces pombe mutants prepared by the method, and a library of gene-targeted heterozygous deletion Schizosaccharomyces pombe mutants. Further, the library is useful in constructing a method and a kit for screening a drug's modes of action.

This invention relates to glucose-sensitive albumin-binding diboron conjugates. More particularly the invention provides novel diboron compounds, and in particular diboronate or diboroxole compounds, useful as intermediate compounds for the synthesis of diboron conjugates.