A composition comprising harvested cotton fiber from cotton cells elongated in a bioreactor, wherein the cotton fiber has a trash content of less than 250 Count/gram (Cnt/g) dry mass is disclosed. Also disclosed are in vitro methods for producing cotton fiber with a low trash content at harvest.

A method of treating a sunflower plant stem is disclosed. The method comprises the steps of: selecting a sunflower plant, removing the flower head and leaves from the stem and preparing the stem for enzymatic treatment by slitting the stem lengthways, removing the inner core from the stem; a) treating the cut stem with a pectinase containing a first buffer solution, for 24 hours with shaking at a pH of 3.8-8.5, and at a temperature of from 20-60 C.; b) removing the stem and washing with water, c) placing the stems into a second buffer solution containing xylanase, at a pH of from 7.0-8.0 and at a temperature of from 37-50 C. for 24 hours with shaking; d) removing the stems and washing with water e) placing the stems into a third buffer solution containing feruloyl esterase, at a pH of 7.2-8.0 containing 0.01-0.05% feruloyl esterase, at a temperature of from 37-50 C. for up to 24 hours, f) removing the stems and washing with water, g) pressing the stems to remove unwanted plant matter.

A method of treating a sunflower plant stem is disclosed. The method comprises the steps of: selecting a sunflower plant, removing the flower head and leaves from the stem and preparing the stem for enzymatic treatment by slitting the stem lengthways, removing the inner core from the stem; a) treating the cut stem with a pectinase containing a first buffer solution, for 24 hours with shaking at a pH of 3.8-8.5, and at a temperature of from 20-60 C.; b) removing the stem and washing with water, c) placing the stems into a second buffer solution containing xylanase, at a pH of from 7.0-8.0 and at a temperature of from 37-50 C. for 24 hours with shaking; d) removing the stems and washing with water e) placing the stems into a third buffer solution containing feruloyl esterase, at a pH of 7.2-8.0 containing 0.01-0.05% feruloyl esterase, at a temperature of from 37-50 C. for up to 24 hours, f) removing the stems and washing with water, g) pressing the stems to remove unwanted plant matter.

A method for preparing Lyocell fibers from sugarcane bagasse is provided, which includes: crushing sugarcane bagasse to 40-80 mesh to obtain sugarcane bagasse powder; putting the sugarcane bagasse powder into a first solution and obtaining pretreated sugarcane bagasse powder; adding the pretreated sugarcane bagasse powder into a second solution and obtaining sugarcane bagasse cellulose; the second solution includes 15-17.5 mol/L glacial acetic acid, 10-15 g/L sodium hypochlorite, and remaining water; adding the sugarcane bagasse cellulose into an NMMO aqueous solution with a mass fraction of 85-88%, stirring and dissolving in a reaction kettle under a vacuum condition at 90-110 C. for 3-5 hours to obtain a transparent spinning solution, where the sugarcane bagasse cellulose has a mass ration of 6-10%; sending the spinning solution into a spinning system for spinning. The process of the present disclosure is simpler, more environmentally friendly, and can reduce costs.

A method for preparing Lyocell fibers from sugarcane bagasse is provided, which includes: crushing sugarcane bagasse to 40-80 mesh to obtain sugarcane bagasse powder; putting the sugarcane bagasse powder into a first solution and obtaining pretreated sugarcane bagasse powder; adding the pretreated sugarcane bagasse powder into a second solution and obtaining sugarcane bagasse cellulose; the second solution includes 15-17.5 mol/L glacial acetic acid, 10-15 g/L sodium hypochlorite, and remaining water; adding the sugarcane bagasse cellulose into an NMMO aqueous solution with a mass fraction of 85-88%, stirring and dissolving in a reaction kettle under a vacuum condition at 90-110 C. for 3-5 hours to obtain a transparent spinning solution, where the sugarcane bagasse cellulose has a mass ration of 6-10%; sending the spinning solution into a spinning system for spinning. The process of the present disclosure is simpler, more environmentally friendly, and can reduce costs.