The compounds relate to zinc-sensitive fluorescent probes, compositions and methods utilizing the same. Such compounds provide an emission-ratiometric fluorescence response upon binding of an analyte. In some embodiments, compounds can be used for two-photon excitation microscopy or conventional fluorescence microscopy. The compounds described herein can also contain one or more functional groups to improve the emission-ratiometric fluorescence response.

Disclosed is a method for capturing the spatial molecular profiling of a biological mass formed from biological material, comprising the steps of: a) providing a transected biological mass, for example a tumour, the transection exposing at least a portion of the mass; b) providing a solid support of an area at least equalling the area of said portion of the mass; c) transferring biological material from the portion of the mass to the support to provide on the support a two dimensional imprint of the biological material present at the portion of the mass; and d) performing a biological assay of the transferred biological material from different predetermined locations of the imprint in order to determine the spatial molecular profile of the portion of the mass.

A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.

A cytometer includes an avalanche photodiode, a switching power supply, a filter, and voltage adjustment circuitry. The switching power supply includes a feedback loop. The filter is electrically connected between the switching power supply and the avalanche photodiode. The voltage adjustment circuitry adjusts a voltage on the feedback loop based at least in part on a voltage measured between the filter and the avalanche photodiode.

The present disclosure features portable wide field fluorimeter systems, e.g., in the form of low-cost mobile platforms, and methods to perform fluorometric assays to detect a change in fluorescence intensity in liquid samples, e.g., caused by the presence of a target analyte, e.g., a protein, e.g., an enzyme (e.g., β-lactamase) expressed by a target pathogen in a liquid sample in a point-of-care setting. In some implementations, a portable system for detecting a change in fluorescence intensity in a liquid sample includes a microfluidic device, an optical assembly including an emission filter and one or more lenses, and an analyzer device that collects and processes a fluorescent signal for the detection of a target analyte produced by the target pathogen present in the liquid sample.

In a real-time traceability method of a width of a defect based on divide-and-conquer provided by the present invention, through the calibration transfer function, the multidimensional eigenvector analysis technology based on the electromagnetic field simulation database of defect scattered dark-field imaging and the adaptive threshold segmentation method, the real-time traceability of the width of the defect greater than and close to the diffraction limit of the system is performed, respectively. The extreme random tree regression model is trained by multidimensional eigenvector analysis technology based on the multidimensional eigenvectors in the electromagnetic field simulation database of the defect scattered dark-field imaging. The present invention solves the problems that the width of the defect in defect detection is difficult to be accurately measured in real time, and the conventional image processing algorithm is difficult to accurately identify the width of the defect close to the diffraction limit of the system.

A miniature Fourier transform mid-infrared (FT-MIR) spectrometer is provided. The FT-MIR includes a metasurface IR source to emit radiation when heated, a microelectromechanical (MEMS) interferometer, and a metasurface microbolometer to measure an interferogram from the MEMS interferometer, wherein the miniature FT-MIR spectrometer is less than about 20 mm in outer diameter.

Apparatus and associated methods relate to a system for detecting foreign object debris ingested into an aircraft engine. The system detects such foreign object debris by projecting a beam of light over an inlet of the aircraft engine. When foreign object debris is ingested into the aircraft engine, it intercepts the beam of light and scatters a back-scattered portion of the intercepted beam of light. An optical detector is configured to detect the back-scattered portion of the intercepted beam of light. A processor is configured to determine whether foreign object debris is ingested by the aircraft engine based on a comparison of a threshold value with a signal indicative of the back-scattered portion generated by the optical detector.

This invention shows a system and method for phenotype characterization of agricultural crops, comprising at least one support device which can be reconfigured in an autonomous or controlled remote manner. It includes a central embedded microcontroller connected to atmospheric sensors located in the upper body, a microcontroller connected to a multi-spectral camera located at the distal end of the arm. The central microcontroller is connected to a base microcontroller that receives signals from soil sensors. A solar panel provides the energy source to a regulating unit that powers the microcontrollers. The system contains a communication unit that includes a router wirelessly connected to Internet.

A high-throughput optofluidic device for detecting fluorescent droplets is disclosed. The device uses time-domain encoded optofluidics to detect a high rate of droplets passing through parallel microfluidic channels. A light source modulated with a minimally correlating maximum length sequences is used to illuminate the droplets as they pass through the microfluidic device. By correlating the resulting signal with the expected pattern, each pattern formed by passing droplets can be resolved to identify individual droplets.