Provided is a method for quantitating a saccharide in a liquid sample. The method comprises incubating the liquid sample with 2,3-naphthalenediamine in the presence of iodine to allow a naphthimidazole group to be linked to the saccharide to obtain a first mixture; obtaining an .sup.1H-NMR spectrum of the first mixture; and comparing, in said .sup.1H-NMR spectrum, the intensity or integral of a proton signal corresponding to the saccharide to the intensity or integral of a proton signal corresponding to an internal standard present in the first mixture.

A diagnostic assay strip includes a first layer that includes an iron mobile labelled specific binding partner that will bind to and iron biomarker from a sample and produce an iron complex and a vitamin A mobile labelled specific binding partner that will bind to a vitamin A biomarker from the sample and produce a vitamin A complex. A second layer includes iron and vitamin A test regions, and a control region. The iron test region has immobilized specific binding partners that will bind to the iron complex. The vitamin A test region has immobilized vitamin A biomarker that will bind to vitamin A mobile labelled specific binding partner, which is not bound to the vitamin A biomarker, passing from the first layer to the second layer. The control region has a moiety which will non-specifically bind to and immobilize the iron and vitamin A labelled specific binding partners. Methods of using the diagnostic assay strip are also discussed.

The present disclosure relates to a method for quantifying sugar appearing in a biological fluid of a subject, the method comprising: (a) obtaining a biological sample from a subject, who has ingested one or more sugars, over a period of 0 to 24 hours after the ingesting; and (b) measuring the amount of the one or more sugars in the biological sample with an organoborane compound coupled to a fluorophore. Disclosed are also assays and kits for performing the above methods.

The present disclosure provides, inter alia, genetically encoded recombinant peptide biosensors comprising analyte-binding framework portions and signaling portions, wherein the signaling portions are present within the framework portions at sites or amino acid positions that undergo a conformational change upon interaction of the framework portion with an analyte.

The present disclosure provides chalcogenopyrylium compounds, composite nanostructures comprising the chalcogenopyrylium compounds, and methods of using the compounds and/or composite nanostructures. For example, composite nanostructures comprising the chalcogenopyrylium compounds are used in imaging applications. The present disclosure provides chalcogenopyrylium compounds having the following structure where each E is, at each occurrence in the compound, independently charged or neutral and is independently selected from S, Se, 0, or Te, wherein at least one E is S or Se; each R1 is, at each occurrence in the compound, independently selected from the group consisting of —H, Ci-s alkyl group, halo group, —CN, aryl group, and heteroaryl group and adjacent R1 groups can combine to form C5ss aryl groups, each R2 is, at each occurrence in the compound.

The invention, provides a method, apparatus and system for separating blood and other types of cellular components, and can be combined with holographic optical trapping manipulation or other forms of optical tweezing. One of the exemplary methods includes providing a first flow having a plurality of blood components; providing a second flow; contacting the first flow with the second flow to provide a first separation region; and differentially sedimenting a first blood cellular component of the plurality of blood components into the second flow while concurrently maintaining a second blood cellular component of the plurality of blood components in the first flow. The second flow having the first blood cellular component is then differentially removed from the first flow having the second blood cellular component. Holographic optical traps may also be utilized in conjunction with the various flows to move selected components from one flow to another, as part of or in addition to a separation stage.

This disclosure comprises devices and methods for determining the identity of individual protein molecules in a complex mixture.

The presently disclosed subject matter provides methods, reporter gene constructs, and kits for using prostate-specific membrane antigen (PSMA) as an imaging reporter to image a variety of cells and tissues.

The present disclosure provides, inter alia, genetically encoded recombinant peptide biosensors comprising analyte-binding framework portions and signaling portions, wherein the signaling portions are present within the framework portions at sites or amino acid positions that undergo a conformational change upon interaction of the framework portion with an analyte.

The present disclosure provides metal-containing (MC) semiconducting (SC) Pdots (MC-SC-Pdots) with beneficial functionalities in both cellular imaging and manipulation, among other applications. The Pdots comprise at least one nanoparticle comprising at least one metal, and a semiconducting polymer associated with the nanoparticle.