Provided herein are methods of using photocleavable labels for multiplex and serial antigen detection. The methods comprise detecting the presence of photocleavable labels, which are conjugated through functional linkers to antigen-binding complexes, which in turn non-covalently bind to antigens. The presence of a photocleavable label is indicative of the presence of an antigen specifically or selectively bound by an antigen-binding complex. Also provided are apparatuses for using photocleavable labels for multiplex and serial antigen detection.

A first aspect of the present invention is directed to a method for the detection of a compound of interest in a microfluidic system. A second aspect of the present invention relates to the use of the method according to the first aspect for monitoring a biological event. A further aspect of the present invention is directed to a microfluidic system and the use thereof for carrying out the method according to the first aspect.

The invention discloses a detection kit for a quaternary ammonium compound having a γ-carboxyl group. The kit includes a fluorescent compound having a fluorophore. The fluorophore of the fluorescent compound is used for forming a derivative with the γ-carboxyl group of the quaternary ammonium compound by an S.sub.N2 nucleophilic substitution reaction. The kit further includes a polar aprotic solvent used for accelerating the S.sub.N2 nucleophilic substitution reaction.

The invention relates to an activated form of procaine, and the use of the activated procaine, or salts or solvates thereof, to label amine-containing compounds, such as N-glycans, amine-containing amino acids, amine-containing peptides, amine-containing proteins, or other amine-containing compounds in a sample. Use of activated procaine as a label allows for sensitive detection of compounds labeled with it both by fluorescence and by mass spectrometry.

This disclosure provides a potency assay for evaluating the potency of facilitating cells and/or alpha beta TCR+ T cells.

Provided are: a coloring cellulose microparticle enabling false positive to be significantly reduced while maintaining a high detection sensitivity; and an immunochromatographic diagnostic kit using the same. The present invention provides: a coloring cellulose microparticle characterized in that an average particle diameter is 60-900 nm, a coloring intensity is 1.0-10.0, and a hydrophilic layer is provided on the surface of microparticle, and a carboxyl group is introduced onto the surface of the microparticle with a spacer therebetween; a structure in which a ligand is covalently bonded to the carboxyl group of said coloring cellulose microparticle; and an immunochromatographic diagnostic kit including said structure.

The present invention relates, in part, to methods and kits for rapidly determining antimicrobial susceptibility of microorganisms.

The present disclosure provides methods for molecular neighborhood detection of molecules, such as by iterative proximity ligation or split-and-pool methods for obtaining positional information.

The present disclosure provides methods, systems, and compositions for parallel processing of nucleic acid samples. Methods and systems of the present disclosure comprise the use of sample-specific barcode sequences, which facilitate the multiplexing of samples, detection of discrete cell populations within a pooled population, and detection of partitions comprising more than one cell.

A signal of fluorescence emitted from a fluorescent particle of a pathological specimen can be increased in sensitivity and be stabilized, thereby resulting in an enhancement in retrieval accuracy of information from a fluorescence image. A pathological specimen including a tissue section subjected to a treatment (immunostaining/FISH staining treatment) for fluorescence-labeling of an objective biomaterial with a fluorescent particle observable in a dark field, based on an immunostaining or FISH method; a packed layer with which the tissue section is covered; and a protection layer with which the packed layer is covered; wherein the refractive indexes of the fluorescent particle, the packed layer and the protection layer (measurement wavelength: 589 nm; measurement temperature: 20 C.; in all) satisfy the conditions of Expressions (1) and (2):
|n1n2|0.20Expression (1)
|n2n3|0.15Expression (2) n1: refractive index (fluorescent particle) n2: refractive index (packed layer) n3: refractive index (protection layer).