Disclosed is an indirect enzyme-linked immunosorbent assay (ELISA) detection kit based on p30 protein and p22 protein of African swine fever (ASF) p30 and p22, belonging to the technical field of animal biological products manufacturing. The indirect ELISA detection kit includes the p30 protein with an amino acid sequence as shown in SEQ ID NO: 2 and the p22 protein with an amino acid sequence as shown in SEQ ID NO: 4.

Embodiments may include a rapid test device that provide rapid detection of substances, including those involved in pathogen infection, for example, using Microscale Affinity Chromatography (MAC), indirect ELISA, and optical molecular sensing technology. For example, in an embodiment, a system may comprise a cartridge comprising a first chamber configured to receive a test sample including a target substance, the first chamber pre-filled with micromagnetic particles treated so as to bind to the target substance, and a first reservoir pre-filled with at least one reagent labeled with at least one fluorescent compound, the reagent adapted to bind to micromagnetic particles that are bound to the target substance, and an apparatus comprising a light source disposed so as to excite the least one fluorescent compound with a light and an optical sensor disposed so as to detect an emitted spectrum of light from the excited least one fluorescent compound.

The invention provides devices and methods for detecting viruses, bacteria, and other analytes of interest in a fluid sample. The fluid sample flows through a first microfluidic channel to a nanoporous or microporous membrane on which are disposed ligands, such as antibodies, specific for the analyte. If the analyte of interest is captured by the ligand, it clogs the pores of the membrane, preventing the fluid sample from passing through the membrane and diverting the fluid into a second channel. Detecting movement of the fluid sample in the second channel signals the presence of the analyte in the fluid sample, while failure of the fluid sample to move in the second channel signals absence of the analyte in the fluid sample.

Air flow systems, devices and methods for monitoring airborne agents include airborne agent collectors. Airborne agent collectors for collecting and detecting the presence and/or identification of an airborne agent(s) include a soluble and hydrophilic polycaprolactone (PCL) that has been treated with a base having a pH greater than 8 and in some embodiments, also treated with a neutralizing agent for increasing hydrophilicity. Detection and identification of airborne agents captured by an airborne agent collector can be performed using any suitable analytical protocols. Such protocols include nucleic acid assays, protein assays, and bioassays. The airborne agent collectors can be used for the detection and identification of nucleic acid from cells or organisms of any type in fixed structures and in mobile, portable devices or machines.

The present invention relates to novel strains of avian reovirus that were isolated from clinical cases of viral arthritis/tenosynovitis in chickens in the southeast United States. The invention is directed to these novel group 1 and group 2 avian reoviruses, diagnostic assays using antibodies and/or nucleotide- or amino acid-specific components of such viruses, such as the S1 gene encoding the sigma C protein, and to vaccines that protect chickens from disease caused by such viruses.

The present invention relates to methods of using bacterial strain 1687A6 in detecting, diagnosing and treating disease or disorders of the GI tract. The present invention also relates to modulating the immune responses of an individual by inducing Th17 cell differentiation, proliferation, or accumulation. Further, the invention relates to therapeutic compositions containing strain 1687A6 or compounds derived from it and methods for treating disease in a subject using such compositions.

An method of malaria detection includes providing an interdigitated sensor having a first electrode with a plurality of fingers and a second electrode with a plurality of fingers, interspersed and spaced apart from the fingers of the first electrode. A quantity of blood to be tested is received on the sensor. The blood is to be dried and is measured for at least one measurable characteristic, including a resistance of the blood. The presence of malaria infected blood is determined based on a comparison between the measurable characteristics and a predetermined threshold related to the measurable characteristics.

The present invention relates to a novel antibody against HERV-K envelope that targets a conserved region not affected by glycosylation or by native conformation, and its use in diagnostics and/or is therapy.

A method for screening substances for their ability to reduce malodours from emanations from an animal, said method comprising determining the effect of said substances on the C-S lyase activity of bacteria that emit volatile sulphuric compounds (VSCs), by contacting a test substance with a sample comprising said bacteria or a supernatant obtainable from a culture of said bacteria in the presence of a substrate for a C-S lyase, detecting the levels of thiol production from said bacteria, and comparing the results with those obtained from similar bacteria in the absence of said substance.

Novel means that enables detection of the monocytogenes bacterium alone distinctly from other bacteria belonging to the genus Listeria with sufficiently high accuracy is disclosed. The present inventors intensively analyzed the genome of the monocytogenes bacterium to identify two genes (the lmo0084 gene and the lmo2736 gene) as target regions with which the monocytogenes bacterium can be specifically detected distinctly from other bacteria belonging to the genus Listeria utilizing a nucleic acid amplification method. By a further intensive study of the base sequences of these two genes, primer setting regions for highly accurate, specific detection of the monocytogenes bacterium alone were identified, and preferred particular examples of PCR primer sets, LAMP primer sets, and real-time PCR primer-probe sets were established.