Methods and system for identification of dimer species using online chromatography and electrospray ionization mass spectrometry are provided. Also provided are methods and system for quantitation of heterodimer species using immunoprecipitation and liquid chromatography-mass spectrometry.

Disclosed are methods, systems, and computer program products for using liquid chromatography/tandem mass spectrometry (LC-MS/MS) for the analysis of endogenous biomarkers, such as 11-oxo androgens, in a sample. The 11-oxo androgens may comprise at least one of 11-hydroxyandrostendione (11OHA), 11-hydroxytestosterone (11OHT) or 11-ketotestosterone (11KT). More specifically, the methods, systems, and computer program products are described for detecting and quantifying the amount of an 11-oxo-androgen in a sample.

The present invention relates to a method for determining whether or not a subject has a parkinsonian condition, comprising determining the concentration of 2-hydroxypyridine in a sample obtained from the subject, wherein the subject is determined to have, or to be at risk of having the parkinsonian condition, if the determined concentration of 2-hydroxypyridine is increased compared to a control. The present invention further also relates to a method for monitoring the progression of a parkinsonian condition in a subject diagnosed with the parkinsonian condition and a method for assessing the efficacy of treatment of a parkinsonian condition in a subject diagnosed with the parkinsonian condition as well as a kit-of-parts for determining whether or not a subject has a parkinsonian condition.

Disclosed herein are mass spectrometry sample substrates. Also disclosed herein are mass spectrometry sample strips and cartridges that include a solid phase extraction (SPE) element. The mass spectrometry sample substrates, sample strips, and cartridges can be used in paper spray mass spectrometry to detect and quantify one or more analytes present in a biological sample. Also disclosed are methods for collecting and concentrating one or more analytes from a biological sample, as well as for storing a biological sample that includes one or more analytes. Methods for analyzing the one or more analytes from the biological sample are also provided.

A sequencing method, system and kit of low molecular weight heparin (LMWH) oligosaccharides are provided. The sequencing method includes: a sample preparation step: isolating or preparing a group of LMWH oligosaccharide mixture samples; a sample treatment step: performing complete enzymatic digestion and nitrous acid degradation on the LMWH oligosaccharide mixture samples to obtain an enzymatically digested eight-common-heparin-disaccharide array, a 3-O-sulfate group array, a 1,6-anhydro structure array, a nitrous acid degradation array, respectively; a data processing step: obtaining a disaccharide isomeric unit array according to the enzymatically digested eight-common-heparin-disaccharide array and the nitrous acid degradation array; a sequence database building step: building a sequence database according to the degree of polymerization of the oligosaccharide mixture, the disaccharide isomeric unit array, the 3-O-sulfate group array, and the 1,6-anhydro structure array; and a specific result output step: screening the sequence database according to input qualification information and then outputting a specific result file.

Systems and methods for determining regions of proteins that contribute to self-association of the protein are provided. Methods for modifying the self-association of concentrated protein formulations are also provided.

This document relates to methods and materials for detecting premalignant and malignant neoplasms. For example, methods and materials for determining whether or not a stool sample from a mammal contains nucleic acid markers or polypeptide markers of a neoplasm are provided.

Real-time search (RTS) for mass spectrometry is described. In one instance, precursor ions generated from a sample are introduced into a mass spectrometer during an introduction period. The precursor ions are fragmented to form product ions, and a mass spectrum is acquired. During the introduction period, scores indicating the similarity between the mass spectrum and a candidate mass spectrum are identified. Based on the distribution of the scores, an action is performed.

Methods are described for measuring the amount of one or more of vitamin A, α-tocopherol, and the combination of β-tocopherol and γ-tocopherol in a sample. More specifically, mass spectrometric methods are described for detecting and quantifying one or more of vitamin A, α-tocopherol, and the combination of β-tocopherol and γ-tocopherol in a sample.

The present disclosure is directed to methods reagents and kits for solid phase extraction, derivatization with crown ether containing derivatizing agents, and mass spectrometry of the derivatized analytes.