Molecularly Imprinted Polymers (MIPs) are utilized to detect diseases and minimize false negative/positive scenarios. MIPs are implemented on a nano-electric circuit in a biochip where interactions of MIPs and an Antigen/Antibody (AG/AB) are detected, and disease specific biomarkers diagnosed. Biomarker detection is achieved with interdigitated gold electrodes in a biochip's microchannel. Capacitance changes due to biomarker interaction with AG/AB electrode coating diagnose diseases in a microfluidic environment. Biofluid passes through the microchannel and exposed to the nanocircuit to generate a capacitance difference and diagnose any specific disease in the biofluid sample. Blood capillary flow in a microchannel curved section experience centrifugal forces that separate liquid from solid. Various blood densities and segments experience different centrifugal effects while flowing through the curved section so serum is separated from various solid matter without using external devices.

A chemical analysis apparatus that quantitatively determines an object of detection rapidly with high sensitivity, and a pretreatment apparatus and a chemical analysis method used for the chemical analysis apparatus, are provided. The chemical analysis apparatus includes a pretreatment unit that accommodates a molecularly imprinted polymer capable of capturing a polar group-containing molecule included in a specimen. A quantification unit quantitatively determines a component included in the specimen that has been passed through the pretreatment unit.

Embodiments of templated polymeric materials capable of binding lysophosphatidic acids (LPAs) are disclosed. Methods of making and using the templated polymeric materials also are disclosed. The disclosed templated polymeric materials are molecularly imprinted polymers that bind LPAs and facilitate the production of lysophosphatidic acid-enriched samples, for instance through extraction of lysophosphatidic acids from biological samples, such as plasma or serum samples.

The subject invention provides chemical compositions and synthesis strategies to create molecularly imprinted polymers (MIPs) via sol-gel processes. In a specific embodiment, the subject invention utilizes a(n) organic, inorganic, or metallic template analyte to create a hybrid organic-inorganic or inorganic three-dimensional network possessing cavities complementary to the shape, size, and functional orientation of the template molecule or ions. The subject invention further pertains to the use of the novel MIPs as selective solid phase extraction (SPE) sorbents for pre-concentration and clean-up of trace substances in biological and environmental samples. Synthesis of other molecularly imprinted polymers with environmental, pharmaceutical, chemical, clinical, toxicological, and national security implications can be conducted in accordance with the teachings of the subject invention.

A molecularly imprinted polymer comprises at least one recognition site that is complementary to a template molecule consisting of an amino acid sequence corresponding to a subsequence of the receptor binding domain of SARS-CoV-2 spike protein, wherein the amino acid sequence is no more than 50 amino acids in length and comprises a sequence selected from (i) NSNNLDSKVGG, (ii) NYNYLYRLFRKS, (iii) YRLFRKSNLKPF, (iv) STEIYQAGSTPC, (v) CNGVEGFNCYF, (vi) GSTPCNGVEGF, (vii) CYFPLQSYGFQP, (viii) GFQPTNGVGYQ and (ix) LQSYGFQPTNG. A method of preparing the molecularly imprinted polymer is also provided. Conjugates comprising the molecularly imprinted polymer and a fluorophore are also provided as are compositions containing the molecularly imprinted polymer and conjugates of the invention. The molecularly imprinted polymer, conjugate and compositions can be used in the detection of SARS-CoV-2.

A double fluorescent particle comprises: a core with a first fluorescence; and a molecularly imprinted polymer (MIP) shell with a second fluorescence; wherein the MIP is an organic polymer comprising elements selected from the group consisting of: C, H, O, N, P, and S; wherein the MIP is adapted to selectively bind to a cell surface structure; wherein the first fluorescence is generated by an entity selected from the group consisting of: a carbon nanodot, an alkaline earth metal fluoride, a dye-doped polymer, a dye-doped stabilized micelle, a P-doti.e. a -conjugated polymer, a quantum dot doped polymer, a rare earth metal ion doped polymer, a dye-doped silica, a rare-earth ion doped silica, and a rare earth ion doped alkaline earth metal fluoride nanoparticle; wherein the second fluorescence is generated by an entity selected from the group consisting of: a dye, a molecular probe, an indicator, a probe monomer, an indicator monomer, and a cross-linker, and wherein the first and second fluorescence differ at least by an emission wavelength and/or by an excitation wavelength.

The present systems and methods relate to devices, methods, and systems for detecting the probability of cancer conditions using Sialic Acid concentrations. The present invention relates to a non-invasive system with diagnostic and treatment capacities that use a unified code that is intrinsic to physiological brain function. Embodiments may provide salivary sialic acid testing for cancer detection, for example, breast and oral cancers. Findings indicate the effectiveness of salivary sialic acid testing for detecting solid cancers, potentially serving as an initial screening tool in laboratory settings before invasive diagnostic procedures are recommended.

Disclosed herein is a molecular imprinted air filter for removing, detecting and/or reporting specific agents and/or molecules and comprising one or more air-permeable layers of molecular imprinted material positioned to contact molecules and/or agents in an airborne, and/or microdroplet-borne environment, a bioactive molecular imprint of a molecule that captures a specific airborne, fluid borne, and/or microdroplet-borne molecule, particle, or agent, and an electronic enhancement.

A nanoscale molecularly imprinted polymer (MIP) thin film for small molecule detection, a method of manufacturing the same, and an electrochemical sensor using the MIP and quantum electrochemical impedance spectroscopy (EIS) are described. A plurality of specific recognition spaces for small molecules of 1000 Da or less are formed in the MIP, a receptor polymer is present at one end of the specific recognition spaces, a redox probe is present in a wire form, and the small molecule is a steroid hormone or a protein. The sensor is useful in point-of-care applications because it exhibits a rapid and reversible small molecule detection ability through a simple electrochemical regeneration process without cumbersome washing and solution replacement steps in the manufacturing process, thereby enabling continuous detection.

The present invention discloses an -synuclein sensing film, a manufacturing method and a use thereof. The -synuclein sensing film comprises a base plate and plural -synuclein sensing polymers polymerized on the base plate. Each of the plural -synuclein sensing polymers has plural -synuclein detection holes on a surface thereof. The plural -synuclein sensing polymers are manufactured by electropolymerization, and the plural -synuclein detection holes are imprinted by an -synuclein peptide. A sample to be tested can be applied to the -synuclein sensing film for detecting the -synuclein therein.