An auto-focusing system is disclosed. The system includes an illumination source. The system includes an aperture. The system includes a projection mask. The system includes a detector assembly. The system includes a relay system, the relay system being configured to optically couple illumination transmitted through the projection mask to an imaging system. The relay system also being configured to project one or more patterns from the projection mask onto a specimen and transmit an image of the projection mask from the specimen to the detector assembly. The system includes a controller including one or more processors configured to execute a set of program instructions. The program instructions being configured to cause the one or more processors to: receive one or more images of the projection mask from the detector assembly and determine quality of the one or more images of the projection mask.

A medical/surgical microscope with two cameras configured to capture two dimensional images of specimens being observed. The medical/surgical microscope is secured to a control apparatus configured to adjust toe-in of the two cameras to insure the convergence of the images. The medical/surgical microscope includes a computer system with a non-transitory memory apparatus for storing computer program code configured for digitally rendering real-world medical/surgical images. The medical/surgical microscope has an illumination system with controls for focusing and regulating the lighting of a specimen. The medical/surgical microscope is configured for real-time video display with the function of recording and broadcasting simultaneously during surgery.

The invention provides an optical super-resolution microscopic imaging system comprising a dichroic beamsplitter for annular parallel light to transmit through; a focusing lens used for converging the annular parallel light transmitted through the dichroic beamsplitter; a confocal pinhole for the annular parallel light after being converged to pass through to filter the annular parallel light; a varifocal lens system for collimating the annular parallel light passing through the confocal pinhole into excited annular parallel light; and a detector for receiving and processing fluorescence emitted by the excited sample, the fluorescence emitted by the excited sample being returned by the same way, and the dichroic beamsplitter separating the fluorescence emitted by the sample from an annular parallel light path and turning the fluorescence to the detector to obtain a super-resolution image of the sample.

A microscope system includes: an enclosed sample chamber for receiving a sample carrier in an examining position in which a sample arranged on the sample carrier is microscopically examinable; an enclosed incubation chamber that is separated from the sample chamber and that receives the sample carrier in at least one storing position; and a sample carrier transfer unit including an enclosed transfer chamber that is connected to the sample chamber by a first opening, and to the incubation chamber by a second opening, and a sample carrier handling device arranged within the transfer chamber for moving the sample carrier between the storing position and the examination position.

For correcting aberration-induced imaging errors of an optical system which includes an objective (14) and an adaptive optic (18), light (5) and a sample (20) are selected such that the light (5), in acting upon the sample (20), reduces a measurement signal (28) from the sample (20), wherein a relative variation of the measurement signal (28) depends on the intensity of the light (5). The measurement signal (28) from a focal area of the optical system in the sample (20) is registered over a first and a later second period of time (38, 37) to determine a first measurement value and a second measurement value. Over a third period of time (39) which overlaps with the first and/or the second period of time, the light (5) is focused into the focal area by means of the optical system. A measure value for the relative variation of the measurement signal (28) is determined from the first and the second measurement values and used in controlling the adaptive optic (18) as a metric to be optimized.

In some embodiments, a method provides a live view mode without scanning a micro optical element array in which successive image(s) are generated, and optionally displayed, that comprise image pixels that represent sample light received from micro optical elements in an array for different, spatially distinct locations in a sample. Images can be of a useful size and resolution to obtain information indicative of a real time sample state. A full image acquisition by scanning a micro optical element array may be initiated when a sample has sufficiently (self-) stabilized. In some embodiments, a method provides images including a stabilization index without scanning a micro optical element array. A stabilization index that represents an empirically derived quantitative assessment of a degree of stabilization may be determined (e.g., calculated) for sample light received from for one or more micro optical elements each represented by one or more image pixels in an image.

Methods and systems are provided for a microscope assembly. In one example, the microscope assembly include an objective arranged at a top of a plate and aligned with a first side of the plate and a tube lens positioned below the objective along the first side of the plate and spaced away from the objective. The assembly further includes a laser auto-focus oriented parallel with a height of the plate and a light source coupled to a central region of the front face of the plate, between the tube lens and the laser auto-focus.

A method for viewing a microscopy specimen is described. The method includes receiving a request to change a field of view of an optical microscope system that images the microscopy specimen. In response to the request, a current field of view is automatically changed to a new field of view. Parameters of the optical microscope system are automatically adjusted to align an illumination plane of a light sheet of the optical microscope system and a detection plane of the optical microscope system. The adjustment of parameters to align the illumination plane with the detection plane is based at least on precalibrated parameters that correspond to the new field of view, the illumination path objective, and the detection path objective.

A method for the microscopy scanning of a specimen. An immersion medium is used between a slide and a microscope objective, said immersion medium wetting a surface of the slide, and the microscope objective being relatively displaced over the surface of the slide for imaging. The surface is provided with a coating which repels the immersion medium.

A method for imaging a sample using a light-sheet microscope includes illuminating the sample from two different illumination directions using two light sheets, which have different polarization states and are superimposed on one another in a coplanar manner in a target region of the sample. An image of the illuminated target region is generated using an imaging optical unit of the light-sheet microscope. An interference pattern is generated using the two light sheets in the illuminated target region, whereby an image modulation corresponding to the interference pattern is applied to the image of the target region. The image modulation is evaluated. The illuminated target region is aligned in dependence on the evaluated image modulation in relation to a focal region of the imaging optical unit.