A holographic microscopy characterization (HMC) process for utilizing holographic video microscopy to provide an efficient, automated, label-free method of accurately identifying cell viability. Optical properties of a sample of cells are determined by HMC. The optical properties are compared to known samples or compared over time to observe changes in the optical properties, enabling identification of cells as viable or not viable, or as extra-cellular or degraded cellular materials.

A 3D optical microscope device of a small form factor optical system is disclosed. A transmission optical system device comprises a first lens having a left side disposed in contact with an input plane, and a second lens having a right side disposed in contact with a rear focal plane and disposed at a position spaced apart by a focal length of the first lens. The first lens and the second lens Fourier-transform a light signal incident on the input plane and output the transformed signal to the rear focal plane.

A holographic imaging device and method realizes both a transmission type and a reflection type, and also realizes a long working distance wide field of view or ultra-high resolution. Object light emitted from an object, sequentially illuminated with parallel illumination light whose incident direction is changed, is recorded on a plurality of object light holograms for each incident direction using off-axis spherical wave reference light. The reference light is recorded on a reference light hologram using in-line spherical wave reference light being in-line with the object light. An object light wave hologram and its spatial frequency spectrum at the object position are generated for each incident direction using each hologram. A synthetic spectrum which occupies a wider frequency space is generated by matching each spectrum in the overlapping area, and a synthetic object light wave hologram with increased numerical aperture is obtained thereby.

A holographic display method and a holographic display system are provided. The holographic display method includes: obtaining target scene information; recognizing human face area information of the target scene information; confirming a pupil position of the human face area information; calculating a lateral viewing angle between each pixel area and each pupil position in a display panel and a driving voltage value of each pixel area at the lateral viewing angle corresponding to each pixel area; and applying the driving voltage value to the corresponding pixel area.

A laser beam (L50) generated by a laser light source (50) is reflected by a light beam scanning device (60) and irradiated onto a hologram recording medium (45). On the hologram recording medium (45), an image (35) of a scatter plate is recorded as a hologram by using reference light that converges on a scanning origin (B). The light beam scanning device (60) bends the laser beam (L50) at the scanning origin (B) and irradiates the laser beam onto the hologram recording medium (45). At this time, scanning is carried out by changing a bending mode of the laser beam with time so that an irradiation position of the bent laser beam (L60) on the hologram recording medium (45) changes with time. Regardless of an irradiation position of the beam, diffracted light (L45) from the hologram recording medium (45) produces a reproduction image (35) of the scatter plate on the spatial light modulator (200). The modulated image of the spatial light modulator (200) is projected onto a screen (400) by a projection optical system (300).

The invention relates to novel compounds which are especially suitable for use as writing monomers in holographic media. The invention further provides a photopolymer and a holographic medium comprising the inventive compounds, and an optical display, a security document and a holographic optical element comprising an inventive holographic medium.

A method for three-dimensional imaging of a sample (302) comprises: receiving (102) interference patterns (208) acquired using light-detecting elements (212), wherein each interference pattern (208) is formed by scattered light from the sample (302) and non-scattered light from a light source (206; 306), wherein the interference patterns (208) are acquired using different angles between the sample (302) and the light source (206; 306); performing digital holographic reconstruction applying an iterative algorithm to change a three-dimensional scattering potential of the sample (302) to improve a difference between the received interference patterns (208) and predicted interference patterns based on the three-dimensional scattering potential; wherein the iterative algorithm reduces a sum of a data fidelity term and a non-differentiable regularization term and wherein the iterative algorithm includes a forward-backward splitting method alternating between forward gradient descent (108) on the data fidelity term and backward gradient descent (110) on the regularization term.

The present invention discloses a method for detecting a microstructure of a functionally graded material based on digital acousto-optic holography, including the following steps: excite a sample with an ultrasonic wave; record a light wave; form a single tomographic acousto-optic hologram; perform numerical reconstruction of phase information, and perform global detection. The present invention uses an acoustic-optic modulation device to modulate a laser light source of a laser of a laser device to form two light waves of different frequencies. The two light waves each constitute a Mach-Zehnder interference system to record reflection wave information and transmission wave information of an ultrasound, and are finally combined and recorded in the same hologram to form the single tomographic acousto-optic hologram.

A light irradiation device includes a Gaussian beam output unit for outputting light having a light intensity distribution that conforms to a Gaussian distribution, a spatial light modulator for receiving the light and modulating the light by presenting a CGH, an optical system for converging the modulated light, and an amplitude mask arranged on at least one of an optical axis between the Gaussian beam output unit and the spatial light modulator and an optical axis between the spatial light modulator and the optical system. The amplitude mask has a circular-shaped first region centered on the optical axis and an annular-shaped second region that surrounds the first region. Transmittance in the second region continuously decreases as a distance from the optical axis increases.

The current invention concerns a sample vial for receiving a liquid cell sample, to be used in conjunction with a digital holographic microscope (DHM), said sample vial comprises at least two compartments in fluid connection with one another, said compartments comprising at least one pair of screening surfaces, said screening surfaces are essentially flat; and characterized in that the distance between the pair of screening surfaces of the second compartment is smaller than the distance between the pair of screening surfaces of the first compartment. In a second and third aspect, the current invention pertains to a method and system for analyzing a liquid cell sample by DHM, employing the sample vial of the current invention.