Provided herein is a method for sequence analysis that comprises analyzing PCR reactions that each contain different portions of the same sample, wherein at least some of the primer pairs are in more than one PCR reaction and at least one of the PCR reactions contains some but not all of the primer pairs of the other reaction(s).

A DNA construct comprises multiple units sequentially attached one to the other, wherein a unit comprises: a segment; an index attached to one end of the segment; an identifier attached to another end of the segment; an introducer attached to a 5′-end of either the index or the identifier; and a closure attached to a 5′-end of a remaining either identifier or index. A method for preparing the DNA construct and a method for analyzing a sequence of the DNA construct, as well as various embodiments thereof, are disclosed herein.

The present invention provides compositions and methods that can be used to identify an antigen or epitope region of an antigen specific for a disease or other condition. Such methods incorporate k-mer binding statistics to serum antibody from condition and control cohort samples to predict the suitability of antigen sequences identified as relevant to the disease or condition as antigen markers. Also disclosed herein are systems for performing the same.

The present invention provides compositions and methods that can be used to identify an antigen or epitope region of an antigen specific for a disease or other condition. Such methods incorporate k-mer binding statistics to serum antibody from condition and control cohort samples to predict the suitability of antigen sequences identified as relevant to the disease or condition as antigen markers. Also disclosed herein are systems for performing the same.

A system and method for accurate determination of sequence variants from noisy sequencing data, including single nucleotide variants and structural variants of the internal tandem duplication type. This system expands the utility of inexpensive sequencing instruments which stream relatively high-error output sequences in real time, such that they may be used in high-stakes contexts, such as clinical cancer care. An example application is Acute Myeloid Leukemia (AML), where healthcare providers may need to make decisions in hours, is provided.

A system and method for accurate determination of sequence variants from noisy sequencing data, including single nucleotide variants and structural variants of the internal tandem duplication type. This system expands the utility of inexpensive sequencing instruments which stream relatively high-error output sequences in real time, such that they may be used in high-stakes contexts, such as clinical cancer care. An example application is Acute Myeloid Leukemia (AML), where healthcare providers may need to make decisions in hours, is provided.

Provided are a method of detecting chromosomal aneuploidy of a targeted fetal chromosome, and a computer-readable medium having recorded thereon a program to be applied to performing the method. According to the present disclosure, fetal chromosomal aneuploidy may be non-invasively and prenatally diagnosed with excellent sensitivity and specificity.

Provided are a method of detecting chromosomal aneuploidy of a targeted fetal chromosome, and a computer-readable medium having recorded thereon a program to be applied to performing the method. According to the present disclosure, fetal chromosomal aneuploidy may be non-invasively and prenatally diagnosed with excellent sensitivity and specificity.

Biosensor including an array of reaction sites and corresponding light sensors may experience crosstalk in which photons from one reaction site are detected by neighbors of its corresponding light sensor, and such crosstalk may be corrected using sharpening kernels corresponding to the sensors in the array. Such sharpening kernels may be derived from point spread functions, which may be determined in real time analysis based on images captured during sequencing.

Biosensor including an array of reaction sites and corresponding light sensors may experience crosstalk in which photons from one reaction site are detected by neighbors of its corresponding light sensor, and such crosstalk may be corrected using sharpening kernels corresponding to the sensors in the array. Such sharpening kernels may be derived from point spread functions, which may be determined in real time analysis based on images captured during sequencing.