A closed type arterial blood collection apparatus is disclosed. The closed type arterial blood collection apparatus includes a saline bag provided at a lower end thereof with a first port and a second port, an A line configured to connect an arterial blood vessel of a patient to the first port, a first three-way valve configured to open and close the A line, a blood collection unit configured to collect arterial blood through the first three-way valve, a second three-way valve provided between the first three-way valve and the first port, the second three-way valve being configured to open and close the A line, a guide tube configured to connect the second three-way valve to the second port, and a fluid conveying means configured to convey a fluid in the guide tube when the A line and the guide tube are connected to each other.