Methods and compositions for synchronizing the time of insemination in swine are described. More particularly, methods are described for synchronizing the time of insemination by administration of a composition comprising a hormone, wherein the swine is inseminated only one time after administration of the hormone, and wherein there is no heat detection.

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).

An IVF system for successfully utilizing spermatozoa separated into X-chromosome bearing and into Y-chromosome bearing population for insemination. The IVF system includes fertilization medium that can shorten the time from insemination to cleavage and a portable incubator for the transportation of maturing oocytes and inseminated oocytes comprising a straw (19) and an incubation element (20) that can be sealed with a cap (22).

The present invention provides highly advantageous and efficient methods for spermatogonial stem cell (SSC) transfer for the generation of animals having valuable traits or genetic background.

The present invention provides highly advantageous and efficient methods for spermatogonial stem cell (SSC) transfer for the generation of animals having valuable traits or genetic background.

The straw comprises a tube (11) extending between a first end and a second end and comprises a gas-permeable, liquid-tight plug, said plug being arranged in the tube (11) in the vicinity of the first end of same and extending between a first end turned towards the first end of the tube (11) and a second end turned towards the second end of the tube (11). Said plug comprises an element (14) impregnated with multivalent cations (22) at least in the vicinity of a first end turned towards the second end of the tube (11). The system comprises the straw and a liquid medium provided to produce, by mixing, in predefined conditions, the liquid substance (21). The medium contains an alginate (23) in solution.

The system comprises a straw and a liquid dilution medium. The straw comprises a tube (11) extending between a first end and a second end and comprises a gas-permeable, liquid-tight plug, said plug being arranged in the tube (11) in the vicinity of the first end of same and extending between a first end turned towards the first end of the tube (11) and a second end turned towards the second end of the tube (11). Said plug comprises an element (14) impregnated with a reagent (22) at least in the vicinity of a first end turned towards the second end of the tube (11). The liquid medium is provided to produce, by mixing, in predefined conditions, the liquid substance (21). The reagent (22) and the medium(27) are configured such that, when the substance (21) comes into contact with the first end of the impregnated element (14), it forms a hydrogel.

The system comprises a straw and a liquid dilution medium. The straw comprises a tube (11) extending between a first end and a second end and comprises a gas-permeable, liquid-tight plug, said plug being arranged in the tube (11) in the vicinity of the first end of same and extending between a first end turned towards the first end of the tube (11) and a second end turned towards the second end of the tube (11). Said plug comprises an element (14) impregnated with a reagent (22) at least in the vicinity of a first end turned towards the second end of the tube (11). The liquid medium is provided to produce, by mixing, in predefined conditions, the liquid substance (21). The reagent (22) and the medium(27) are configured such that, when the substance (21) comes into contact with the first end of the impregnated element (14), it forms a hydrogel.

The present invention provides highly advantageous and efficient methods for spermatogonial stem cell (SSC) transfer for the generation of animals having valuable traits or genetic background.

There are described delivery devices for insertion into a body cavity of a target mammal, the delivery device comprising a resilient frame adapted to receive one or more moulded impregnated masses. The frame, together with the impregnated mass, are insertable, retainable and withdrawable from the intended body cavity. The impregnated mass or masses are attached to the frame to present substantially all of each face of the masses to exposure to a fluid of the body cavity when retained therein.