The present invention relates to a method for promoting production of female embryos in fertilized eggs, comprising steps of: incubating said eggs; generating at least one type of energy with a predetermined energy profile; and simultaneously generating a pre-determined profile of a non-energy parameter by means of an energy generation device, applying both energy profile and the non-energy parameter to the eggs; and, collecting the eggs; wherein said method is non-toxic to the embryos, the application of both the energy profile and the non-energy parameter increases the fraction of females in the hatched chicks.

A novel piezo-driven cell injection system with force feedback overcomes the unsatisfied force interaction between the pipette needle and embryos in conventional position control. By integrating semiconductor strain-gage sensors for detecting the cell penetration force and the micropipette relative position in real time, the developed cell microinjection system features high operation speed, confident success rate, and high survival rate. The effectiveness of the developed cell injection system is experimentally verified by penetrating zebrafish embryos. The injection of 100 embryos are conducted with separate position control and force control. Results indicate that the force control enables a survival rate of 86%, which is higher than the survival rate of 82% produced by the position control in the same control environment. The experimental results quantitatively demonstrate the superiority of force control over conventional position control for the first time.

A novel piezo-driven cell injection system with force feedback overcomes the unsatisfied force interaction between the pipette needle and embryos in conventional position control. By integrating semiconductor strain-gage sensors for detecting the cell penetration force and the micropipette relative position in real time, the developed cell microinjection system features high operation speed, confident success rate, and high survival rate. The effectiveness of the developed cell injection system is experimentally verified by penetrating zebrafish embryos. The injection of 100 embryos are conducted with separate position control and force control. Results indicate that the force control enables a survival rate of 86%, which is higher than the survival rate of 82% produced by the position control in the same control environment. The experimental results quantitatively demonstrate the superiority of force control over conventional position control for the first time.

The sex of embryos in eggs is influenced or controlled through the application of light having selected wavelengths in order to promote the development of embryos of a selected sex. An incubating device is provided having an interior cavity that can be sealed from an outside, and having a plurality of lighting elements in the interior cavity. Eggs are disposed in the interior cavity, and pre-determined environmental conditions are applied to the interior cavity to promote hatching of the eggs. Concurrently with the application of the environmental conditions, the eggs are irradiated according to pre-determined lighting conditions. The lighting conditions include applying light having wavelengths substantially concentrated in selected ranges, such as light wavelengths within the 440-460 nm, or other narrow range.

The sex of embryos in eggs is influenced or controlled through the application of light having selected wavelengths in order to promote the development of embryos of a selected sex. An incubating device is provided having an interior cavity that can be sealed from an outside, and having a plurality of lighting elements in the interior cavity. Eggs are disposed in the interior cavity, and pre-determined environmental conditions are applied to the interior cavity to promote hatching of the eggs. Concurrently with the application of the environmental conditions, the eggs are irradiated according to pre-determined lighting conditions. The lighting conditions include applying light having wavelengths substantially concentrated in selected ranges, such as light wavelengths within the 440-460 nm, or other narrow range.

The present invention relates to a method of improving a reproductive potential of a female mammal using an ultra-weak photon. In the method of improving a reproductive potential of a female mammal using an ultra-weak photon according to the present invention, an ultra-weak photon is in a visible light spectrum but is transmitted as weak light that is not visually recognized so that the ultra-weak photon does not provoke stress and can be irradiated safely for a long time, thereby improving the reproductive potential of female mammals. Unlike an existing material feeding method of improving a reproductive potential, in which a material is supplied wastefully and environmental pollution problems are caused due to tolerance and misuse, the method of improving a reproductive potential of a female mammal is a useful alternative technology that can be used for a long time and ensure the sustainability of livestock industries.

The present invention relates to a method of improving a reproductive potential of a female mammal using an ultra-weak photon. In the method of improving a reproductive potential of a female mammal using an ultra-weak photon according to the present invention, an ultra-weak photon is in a visible light spectrum but is transmitted as weak light that is not visually recognized so that the ultra-weak photon does not provoke stress and can be irradiated safely for a long time, thereby improving the reproductive potential of female mammals. Unlike an existing material feeding method of improving a reproductive potential, in which a material is supplied wastefully and environmental pollution problems are caused due to tolerance and misuse, the method of improving a reproductive potential of a female mammal is a useful alternative technology that can be used for a long time and ensure the sustainability of livestock industries.

The present invention relates to a method of improving a reproductive potential of a male mammal using an ultra-weak photon. The ultra-weak photon according to the present invention is in a visible light spectrum but is transmitted as weak light that is not visually recognized so that the ultra-weak photon does not provoke stress and can be irradiated safely for a long time, thereby improving the reproductive potential of male mammals. Unlike an existing material feeding method of improving a reproductive potential, in which a material is supplied wastefully and environmental pollution problems are caused due to tolerance and misuse, the present invention relates to a useful alternative technology that can be used for a long time and ensure the sustainability of livestock industries.

The present invention relates to a method of improving a reproductive potential of a male mammal using an ultra-weak photon. The ultra-weak photon according to the present invention is in a visible light spectrum but is transmitted as weak light that is not visually recognized so that the ultra-weak photon does not provoke stress and can be irradiated safely for a long time, thereby improving the reproductive potential of male mammals. Unlike an existing material feeding method of improving a reproductive potential, in which a material is supplied wastefully and environmental pollution problems are caused due to tolerance and misuse, the present invention relates to a useful alternative technology that can be used for a long time and ensure the sustainability of livestock industries.

A method of in vitro fertilization wherein the embryo is implanted into the uterus of a female patient at least two, and preferably three to twelve months after the eggs are retrieved from the patient in order to reduce the effect of autoimmune rejection of the embryo by the patient's autoimmune system and increase the probability and success of pregnancy and wherein prior to embryo implantation, the endometrium in the uterus is prepared for embryo implantation by introducing peripheral blood mononuclear cells (PBMCs) into the uterus. The procedure is combined with cryopreservation techniques to preserve the oocytes or the IVF-produced embryos of the patient.