A mobile, self-contained system for enhancing plant growth comprising: a mobile structure comprising a plurality of wheels so that the system can be moved; a vessel supported by the structure; a recirculating pump supported by the structure and exterior to the vessel, the pump having a first discharge line and a second discharge line; a generator supported by the structure; a vessel outlet from a bottom portion of the vessel to the pump; and an aerator for injecting air into the first discharge line; wherein the first discharge line extends from the pump directly into the bottom portion of the vessel, such that the contents of the vessel can be recirculated by the pump from the vessel outlet back into the vessel through the first discharge line and the second discharge line extends from the pump and is adapted for discharging contents of the vessel directly into an irrigation system.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Compositions and methods for controlling pests are provided. The methods involve transforming organisms with a nucleic acid sequence encoding an insecticidal protein. In particular, the nucleic acid sequences are useful for preparing plants and microorganisms that possess insecticidal activity. Thus, transformed bacteria, plants, plant cells, plant tissues and seeds are provided. Compositions are insecticidal nucleic acids and proteins of bacterial species. The sequences find use in the construction of expression vectors for subsequent transformation into organisms of interest including plants, as probes for the isolation of other homologous (or partially homologous) genes. The pesticidal proteins find use in controlling, inhibiting growth or killing Lepidopteran, Coleopteran, Dipteran, fungal, Hemipteran and nematode pest populations and for producing compositions with insecticidal activity.

Provided are improved compositions and methods for achieving gene therapy in hematopoietic cells and hematopoietic precursor cells, including erythrocytes, erythroid progenitors, and embryonic stem cells. Also provided are improved gene therapy methods for treating hematopoietic-related disorders. Retroviral gene therapy vectors that are optimized for erythroid specific expression and treatment of hemoglobinopathic conditions are disclosed.

Signal sequences useful for targeting proteins and peptides to the surface of endospores produced by Paenibacillus family members and methods of using the same are provided. The display of heterologous molecules, such as peptides, polypeptides and other recombinant constructs, on the spore surface of Paenibacillus family members, using particular N-terminal targeting sequences and derivatives of the same, are also provided.

Signal sequences useful for targeting proteins and peptides to the surface of endospores produced by Paenibacillus family members and methods of using the same are provided. The display of heterologous molecules, such as peptides, polypeptides and other recombinant constructs, on the spore surface of Paenibacillus family members, using particular N-terminal targeting sequences and derivatives of the same, are also provided.

Signal sequences useful for targeting proteins and peptides to the surface of endospores produced by Paenibacillus family members and methods of using the same are provided. The display of heterologous molecules, such as peptides, polypeptides and other recombinant constructs, on the spore surface of Paenibacillus family members, using particular N-terminal targeting sequences and derivatives of the same, are also provided.

The present invention relates to a bio-control method for combating the propagation of phytopathogenic fungi or oomycetes on plants, comprising the application, to the soil and/or to the vegetative apparatus of plants that are infected or are capable of being infected by pathogenic endemic fungal or oomycete strains referred to as type A, of a composition comprising a mixture of at least two strains, referred to as type B, of the same species, said type B strains being non-pathogenic for said plant, sexually compatible with said pathogenic type A strains and characterized in that they exhibit: a) sexual reproduction, b) a sexual phase initiated in non-parasitic mode, c) reproduction according to a heterothallic mode, and d) the existence of special forms or divergent populations within the species, capable of producing, by crossing with the type A strains, descendants that are non-pathogenic or even sterile on said plant of interest, and characterized in that the mixture of non-pathogenic type B strains comprises strains of opposite mating-type signs.

The present invention relates to a bio-control method for combating the propagation of phytopathogenic fungi or oomycetes on plants, comprising the application, to the soil and/or to the vegetative apparatus of plants that are infected or are capable of being infected by pathogenic endemic fungal or oomycete strains referred to as type A, of a composition comprising a mixture of at least two strains, referred to as type B, of the same species, said type B strains being non-pathogenic for said plant, sexually compatible with said pathogenic type A strains and characterized in that they exhibit: a) sexual reproduction, b) a sexual phase initiated in non-parasitic mode, c) reproduction according to a heterothallic mode, and d) the existence of special forms or divergent populations within the species, capable of producing, by crossing with the type A strains, descendants that are non-pathogenic or even sterile on said plant of interest, and characterized in that the mixture of non-pathogenic type B strains comprises strains of opposite mating-type signs.

This invention relates generally to methods of preparing compositions from microorganisms such as cyanobacteria, particularly to preparing compositions with antifungal and/or antibacterial characteristics, and more particularly to preparing cyanobacteria or other microorganism-derived compositions for use in construction materials. Cyanobacteria make various kinds of natural products that may be useful for a variety of applications, including products with antifungal and/or antibacterial activity, and other biologically active compounds (e.g. immunomodulatory, antitumor, antithrombotic, anticoagulant, anti-mutagenic, anti-inflammatory, antimicrobial, and antiviral). Some of the especially useful ones might be antifungals and antibacterials, such as by incorporation or application onto construction materials (e.g. drywall).