A modular cell culture system includes a standalone cell culture subunit with an interior cavity to house a cell culture substrate in a cell culture space, a radial flow manifold below the cell culture space, a fluid inlet to supply fluid to a center of the manifold, and a fluid outlet to remove fluid from the cavity. The cavity is arranged for fluid to flow in from the fluid inlet, then through manifold, then through the cell culture space, and then out through the fluid outlet. The subunit further includes an alignment feature on at least one of a top and a bottom of the standalone cell culture subunit. The manifold can supply fluid at a uniform rate across a width of the cell culture space, and the alignment feature aligns with an alignment feature of another standalone cell culture subunit, such that multiple standalone cell culture subunits are stackable.

A modular and stacked cell culture system that includes a standalone cell culture subunit with an interior cavity to house a cell culture substrate in a cell culture space, a fluid inlet to supply fluid to the cell culture space, and a fluid outlet to remove fluid from the cavity. The cavity is arranged for fluid to flow in from the fluid inlet, then through the cell culture space, and then out through the fluid outlet. The subunit further includes an alignment feature on at least one of a top and a bottom of the standalone cell culture subunit, wherein the alignment feature aligns with an alignment feature of another standalone cell culture subunit, such that multiple standalone cell culture subunits are stackable.

Disclosed herein are scalable, modular bioreactor systems for efficient preparation of cell-based tissues. Also disclosed herein are methods, compositions, and apparatuses for preparing scaffolds.

The present invention has: a step for heating a resin or a metal in a state in which the resin or metal is sandwiched by a mold provided with a projection that is constituted from an elastic body having a heat resistance temperature higher than the temperature at which the resin or the metal softens, and a support body which is larder than the mold and which is softened by heat at a higher temperature than the resin or the metal, while a force is applied to the mold in the direction toward the resin or the metal.

A portable culture test apparatus, comprising: a mounting portion on which a culture dish can be mounted; a vibrating portion that vibrates the mounting portion; a temperature adjusting mechanism that adjusts the temperature of the mounting portion; and an ultraviolet lamp that irradiates the culture dish mounted on the mounting portion with ultraviolet.

Provided herein are systems for continuously measuring oxygen and carbon dioxide gas levels inside a cell culture incubator, comprising an oxygen sensor that uses LED based fluorescence technology to determine the amount of oxygen in the surrounding atmosphere, a carbon dioxide sensor that uses LED based Non-dispersive Infra-Red (NDIR) technology to determine the amount of carbon dioxide in the surrounding atmosphere, and electronics to wirelessly monitor the output from the oxygen sensor and carbon dioxide sensor. Most preferably, the system can be placed inside a cell culture incubator, and sensor readings are pre-compensated for temperature, pressure, and humidity. Also provided herein are methods of using the same.

The invention discloses a first connection unit having a plurality of sensor surfaces and which is adapted to be asepetically connected to a second connection unit mounted e.g. on a flexible bioreactor bag.

The present disclosure provides compositions and methods for producing hydrogel matrix constructs. Methods of using hydrogel matrix constructs for tissue repair and regeneration and for the oxygenation of red blood cells are also disclosed.

An apparatus, that relates to the field of in vitro fertilization, is provided for the combined incubation and vitrification of a biological material. The apparatus can be configured to allow for automatic incubation and vitrification of a viable biological material. Thereby predetermined protocols for handling the biological material can be performed precisely and accurately thus avoiding errors and deviations from the intended protocol, as caused by manual human intervention.

The present approach relates to the design and use of a functionally closed bioreactor designed to immobilize, culture, and mature tissue on a loading platform. The bioreactor may be equipped with sensors for tissue monitoring which in conjunction with stiffness data can provide closed-loop control of tissue maturation. Based on a relationship between cartilage stiffness and tissue maturity, measurements of stiffness can be acquired and used as a surrogate for cartilage maturity without the need for destructive tests.