A feed bag construction is provided comprising a feed bag, a first conduit sealed to the feed bag, a second conduit sealed to the interior of the feed bag for supplying wash reagent to the feed bag and a one piece cap that is removably mounted on the first conduit. The first conduit is provided with a hand operated butterfly valve to open or close the conduit.

Device (1) for the storage and selection of cartridges (20) that are pre-filled with reactive discs to be placed on a support of the type comprising a plurality of magazines (2) each able to store a cartridge (20) of discs, at least one magazine-holding support (3) and, for each support (3), drive means (4) for moving the magazine-holding support (3). The device (1) comprises a chamber (5) configured to house at least one magazine-holding support (3), said chamber (5) being fitted with a cooler (6) and provided with at least one placement opening (7) facing which the drive means (4) for rotating a magazine-holding support (3) are configured to position at least one magazine (2) of said support (3) in what is referred to as the placement configuration of said magazine (2), and the device (1) comprises a drive mechanism (8) driving the movement of the magazine (2) in the placement configuration between a position in which said magazine (2) is distant from and a position in which same is closer to the placement opening (7) of the chamber (5).

Disclosed herein are systems, devices, and methods for sterilization. In some variations, a method of sterilizing a bioreactor system may comprise circulating a sterilant in a bioreactor and an enclosed vessel in fluidic communication with the bioreactor. The enclosed vessel may comprise cell culture media. The sterilant may be circulated for a dwell time sufficient to sterilize at least one of the bioreactor, the vessel, and the cell culture media.

Provided is a medical container from which an accommodated object is easily extracted without being contaminated. A medical container 1 formed by joining together rims of a set of sheet-shaped members 50 and 50 disposed facing each other is provided with: an accommodation part 10 that is disposed at a central part in a width direction WD; and a pair of extending parts 20 that is disposed on one end side in a height direction HD of the accommodation part 10 and extends outward in the width direction from the accommodation part 10. The medical container 1 is preferably provided with partition joining parts 30 that extend from the other end side to the one end side in the height direction HD and partition the accommodation part 10 and the extending parts 20 at boundary portions between the accommodation part 10 and a pair of the extending parts 20.

Embodiments of the present disclosure can include a method for convective intracellular delivery including providing cells and molecules to a microchannel having compressive surfaces, wherein the compressive surfaces define compression gaps having a height of from 20 and 80% of the average cell diameter; and a plurality of relaxation spaces disposed between the compressive surfaces; flowing the cell medium through the microchannel, wherein as the cell medium flows through the microchannel, the plurality of cells undergo a convective intracellular delivery process comprising: compressing the plurality of cells, wherein the compressing causes the plurality of cells to undergo a loss in intracellular volume (V.sub.loss); and passing the plurality of cells to a first relaxation space, wherein the plurality of cells undergo a gain in volume (V.sub.gain) and absorb a portion of the plurality of molecules.

Disclosed is a system for freezing, storing, transporting, and thawing biopharmaceutical product, comprising a stationary equipment and one or more movable pallets, each pallet configured to carry one or more container units, each containing biopharmaceutical product, the stationary equipment comprising a gas circulating unit configured to cause a circulation of cold gas and/or warm gas, from a pull inlet toward a blow outlet, wherein there is provided at least a first side plenum having one or more gas conveying channel therein, configured to be fluidly coupled to the blow outlet of the gas circulating unit, the first side plenum having a plurality of outlet ports directed toward an interior volume of the pallet, wherein the gas circulates substantially in a horizontal direction in the interior volume of the pallet, and wherein a shaking arrangement is provided.

A cell cryopreservation pretreatment operation plate includes a planar operation portion, a first recess for storing a treatment solution, a second recess for storing a treatment solution, a third recess for storing a treatment solution, and a fourth recess for storing discarded solutions. The planar operation portion includes, on a surface thereof, a first circular segment, second circular segment, and third circular segment for placing treatment solutions, an enlarged first circular segment enclosing the first circular segment, an enlarged second circular segment enclosing the second circular segment, and an enlarged third circular segment enclosing the third circular segment.

A container (100) having a first end (102) a second end (104), and a deformable middle portion (106) and methods for manufacturing the container are disclosed. The container has a collapsed configuration and an expanded configuration. The first and second ends are rigid. The container is configured to store cell culture solution powder. Methods and systems (e.g., a dispenser) for creating culture solution using the container are also disclosed.

An automated apparatus for preparing a liquid bioprocess solution includes at least one mixing chamber having a lower port and an upper port for fluid to enter the at least one mixing chamber, an array of tubing for fluid flow within the system, a plurality of valves provided within the tubing, and a mixing controller configured to cause the automated apparatus to perform a series of sequential mixing steps causing the preparation of the liquid bioprocess solution from a dry ingredient. The series of sequential mixing steps include opening a first valve associated with the lower port to provide fluid to the at least one mixing chamber through the lower port, and after a predetermined amount of elapsed time, closing the first valve and opening a second valve associated with the upper port to provide fluid to the at least one mixing chamber through the upper port.

A method for distribution of supports impregnated with antibiotics includes a step of positioning of a plurality of tubular containers, each one containing a plurality of supports impregnated with antibiotics and stacked, in a corresponding plurality of seats of a dispenser. Extraction of at least a first support from a first container is performed using an extractor, the step of extraction being performed in correspondence of an upper end of the first container and comprising a movement of the first support between a first position inside of the first container and a second position extracted from the first container, said first position and said second position lying substantially in correspondence of an upper surface of the dispenser. Collection of the first support from the second position is performed using a collector. Transportation of the first support towards a culture plate by the collector is performed.