Provided are methods of making and using an antimicrobial composition, optionally including adding one or more enzymes to a suspension of lees, wherein the lees was formed by fermenting fruit with yeast and the one or more optional enzymes comprise a protease, a carbohydrase, or a combination of a protease and a carbohydrase; and forming a dried lees by drying the lees. In some examples the fruit includes chardonnay grapes, pinot noir grapes, cabernet franc grapes, or a combination of any two or more of chardonnay grapes, pinot noir grapes, and cabernet franc grapes; the yeast includes one or more Saccharomyces cerevisiae strains of yeast; and drying the lees by heating it. In some examples, trub made from fermenting a grain such as barley is used instead of lees. An antimicrobial composition made as provided may be administered to an animal to inhibit bacterial growth.

The present invention relates to compositions such as cleaning compositions comprising a mix of enzymes. The invention further relates to use of compositions comprising such enzymes in cleaning processes.

Detergent compositions may include endoglucanase variants and methods of using such detergent compositions. The endoglucanase may have an alteration at one or more positions where the variant has at least 60% but less than 100% sequence identity to SEQ ID NO: 2.

The present invention relates to a method for preparing oligosaccharides which can be used among others as food additives to reduce calorie content, to sweeten food products, to increase the fiber content of food products, to improve the texture of food products and to stimulate the gut microbiome bacteria. Furthermore they can be applied in the fields of animal feed, or other applications. More particularly, this invention is directed to a high temperature hydrolysis of xyloglucan polysaccharide to defined xyloglucan oligosaccharides. The invention further relates to oligosaccharide hydrolysates produced with the method of the invention and to the use of said oligosaccharide hydrolysates in human and/or animal nutrition, as prebiotic or other uses. Further provided are novel endoglucanases for use in the method of the invention as well as in other applications.

The disclosure provides methods using mixed substrate cofeeding for bioproduct synthesis, which enables faster, more efficient, and higher yield carbon conversion in various organisms.

Disclosed are carbohydrate binding module variants, as well as variants of a glycoside hydrolase comprising the carbohydrate binding module variants. The variants having glycoside hydrolase activity comprising the carbohydrate binding module variants have improved stability in the presence of a protease, and are useful in detergent applications, such as laundry or dish wash.

The present invention relates to endoglucanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, expression vectors, and recombinant host cells comprising the polynucleotides; and methods of using the variants.

The present invention provides a method of improving the taste of yeast cells including a step of reacting the yeast cells with protease or cellulase, and a quality improving material for food containing yeast cells as an active ingredient in which the taste is improved by the method of improving the taste.

Provided herein is a process for hydrolyzing a cellulosic feedstock to produce sugar. The process comprises introducing a pretreated cellulosic slurry to an inlet region of a plug flow hydrolysis reactor using a slurry introduction device that reduces the axial momentum of the slurry at the surface of the reactor contents. The cellulosic feedstock slurry is hydrolyzed in the plug flow hydrolysis reactor by contacting the cellulosic feedstock with at least cellulase enzymes to produce glucose. Also provided herein is a vertically-oriented, unmixed downflow hydrolysis reactor for hydrolyzing a pretreated cellulosic feedstock slurry which comprises such a slurry introduction device disposed in a top region thereof.

A method for treating a forage to increase digestibility by a ruminant comprises providing a forage for a ruminant comprising a level of ammonia that is from 0.0% to about 1.4% of dry matter (wt/wt). An alkali is applied at a dosage of from about 0.25% to about 0.75% of dry matter (wt/wt) to the forage. At least one exogenous carbohydrase enzyme is applied to the forage after application of the alkali and the forage is subsequently fed to the ruminant.