The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

The present invention relates to isolated polypeptides having protease activity, and polynucleotides encoding the polypeptides. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides.

The present invention relates to a method of producing a recombinant polypeptide a filamentous fungus which is genetically modified to decrease or eliminate the activity of cellulase regulator 2 (CLR2) and to express said recombinant polypeptide. The method further relates to a filamentous fungus Myceliophthora thermophila, which is genetically modified to decrease or eliminate the activity of CLR2 and the use of this filamentous fungus in the production of a recombinant polypeptide.

The present invention relates to polypeptides having mannanase activity, catalytic domains, and carbohydrate binding modules, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding modules.

A method of producing a sugar liquid includes: (A) reacting mannanase with a liquid component obtained by hydrolysis treatment of woody biomass to obtain a saccharified liquid; and (B) filtering the saccharified liquid in Step (A) through a microfiltration membrane and/or ultrafiltration membrane to collect a sugar liquid from a permeate side.

The present invention relates to polypeptides having mannanase activity, catalytic domains, and carbohydrate binding modules, and polynucleotides encoding the polypeptides, catalytic domains, and carbohydrate binding modules. The invention also relates to nucleic acid constructs, vectors, and host cells comprising the polynucleotides as well as methods of producing and using the polypeptides, catalytic domains, and carbohydrate binding modules.

The present invention provides a method for determining carbonyl ratio and/or concentration of oxidized nanofibrillar cellulose in a sample. In accordance with the invention oxidized nanofibrillar cellulose comprised in the sample is enzymatically hydrolyzed into oxidized cellobioses which are specific markers to oxidized nanofibrillar cellulose. The cellobioses may be then analyzed and quantified to reveal the amount of oxidized nanofibrillar cellulose in the sample. A method for determining the concentration of oxidized nanofibrillar cellulose in a sample comprises steps of providing an analytical sample of material comprising oxidized nanofibrillar cellulose; hydrolyzing the analytical sample to breakdown products of oxidized nanofibrillar cellulose in presence of one or more enzymes; subjecting the breakdown products to a separation analysis to reveal the relative amounts of the breakdown products; and determining the concentration of oxidized nanofibrillar cellu-lose.

A method and special complex enzyme for hydrolyzing a galactomannan (GM) to prepare a small-molecule GM and a galactomannan oligosaccharide (GMOS) is provided. The method includes: conducting fermentation with microcrystalline cellulose (MCC) and melibiose as carbon sources and Trichoderma reesei (T. reesei) as an enzyme-producing strain to obtain a supernatant, which is a complex enzyme solution with enzymatic activities of -mannanase and -galactosidase; and directly using the complex enzyme solution for enzymatic hydrolysis of a GM as a substrate to prepare the small-molecule GM and the GMOS.

The present disclosure provides engineered eukaryotic cells comprising a surface displayed fusion proteins comprising a catalytic domain of an enzyme and an anchoring domain of a glycosylphosphatidylinositol (GPI)-anchored protein and methods of use.

The invention provides enzyme granules, comprising an enzyme and one or more spore(s).