The present invention relates to application of a novel signal peptide in L-arginine and its derivatives production from konjac powder, which belongs to the field of gene engineering, enzyme engineering and metabolism engineering. The present invention fused the signal peptide set forth in SEQ ID NO.1 with the -mannanase of Bacillus subtilis CCTCC M 209200, and expressed the fused gene in the strain with high L-arginine yield. The recombinant strain Corynebacterium crenatum CGMCC 0890/p MSPman had advantages on utilizing cheaper konjac powder as substrate, and after fermenting for 96 hours in a 5 L bioreactor, the L-arginine yield reached 45 g/L. Another two recombinant strains were constructed based on Corynebacterium crenatum CGMCC 0890/pMSPman, and after fermenting for 96 hours in a 5 L bioreactor, the L-ornithine yield and L-citrulline reached 23.5 g/L and 26.3 g/L respectively.

The present invention relates to application of a novel signal peptide in L-glutamate and its derivatives production from konjac powder, which belongs to the field of gene engineering, enzyme engineering and metabolism engineering. The signal peptide which mediated secretion of -mannanase was invented, and the recombinant strain with this signal peptide had advantages on utilizing konjac powder to produce related products, and its utilization efficiency of konjac powder, production efficiency, and yield were higher than other signal peptides. The recombinant strain possessing this new signal peptide had advantages on utilizing cheaper konjac powder as substrate to lower the process costs on L-glutamic acid and its high-value-added products.

Disclosed are a mixture of modified polysaccharides and the process of making the modified polysaccharides. Embodiments disclose processing and applying a preparation of the mixture of modified polysaccharides in combination with other bioactive ingredients in aloe, such as amino acids onto the inside surface of a polymer elastomeric flexible article, for example a glove, to enhance the water holding capacity and to regulate pH of the user's skin through effective epidermal penetration.

The present compositions and methods relate to a beta-mannanase from Bacillus hemicellulosilyticus, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).

The present invention concerns a method for decontaminating the starches used as a raw material for the preparation of glucose polymers intended for peritoneal dialysis, the method comprising the following steps: preparing a Waxy corn starch, placing the Waxy starch in suspension at a concentration of between 20 and 40% dry matter in a process water at a pH of between approximately 5 and approximately 6, in particular approximately 5.5, treating the starch suspension with a peracetic acid solution at a concentration equal to or between 100 and 500 ppm, preferably 300 ppm, dewatering the starch, then dissolving in demineralised water adjusted to a pH of between approximately 5 and approximately 6, in particular approximately 5.5 and at a concentration of between 20 and 40% dry matter, increasing the temperature to 107 C., then adding an alpha-amylase for 15 minutes, optionally, treating with an enzymatic preparation having detergent and clarification properties, filtering the suspension on a bed of diatoms, treating with an active carbon having a very high adsorption capacity, of pharmaceutical quality, and of microporous Porosity, treating with a second active carbon of mesoporous porosity, optionally, passing over a macroporous adsorbent polymer resin, having a porosity greater than 100 angstroms, optionally, continuous 5000 Da ultrafiltration, safety filtration through a sterile filter having a porosity of 0.22 m.

The present compositions and methods relate to a beta-mannanase from Bacillus hemicellulosilyticus, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).

The present invention relates to mannanase variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and detergent compositions and methods of using the variants.

Mammalian milk oligosaccharides (MMO) are produced in plants engineered to express recombinant MMO biosynthetic pathways.

The present compositions and methods relate to a beta-mannanase from Mahella australiensis, polynucleotides encoding the beta-mannanase, and methods of make and/or use thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).

The present compositions and methods relate to a beta-mannanase from Paenibacillus polymyxa, polynucleotides encoding the beta-mannanase, and methods of making and/or using thereof. Formulations containing the beta-mannanase are suitable for use in hydrolyzing lignocellulosic biomass substrates, especially those comprising a measurable level of galactoglucomannan (GGM) and/or glucomannan (GM).