The present application relates to improving the production yield of glucose to be prepared by using starch sugar as a raw material and using an Acidothermus sp. derived enzyme. The present application can replace an acid saccharification method, which produces a bitter taste through a hydrolysis reverse reaction, and, compared to a enzymatic saccharification method in which a conventional debranching enzyme is used, has an advantage of enabling costs to be reduced since a relatively high yield can be ensured by reducing side reactions of a saccharification reaction.

An enzymatically produced soluble ?-glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble ?-glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble ?-glucan fiber are also provided.

An isoamylase having improved heat resistance and an industrial method for producing maltose from starch. The isoamylase is an isoamylase consisting of the amino acid sequence represented by SEQ ID NO: 1 or an isoamylase resulting from deletion, substitution, or insertion of one to several amino acid residues in the amino acid sequence represented by SEQ ID NO: 1, wherein at least valine at amino acid number 515 and methionine at amino acid number 570 are mutated to other amino acids.

An enzymatically produced soluble -glucan fiber composition is provided suitable for use as a digestion resistant fiber in food and feed applications. The soluble -glucan fiber composition can be blended with one or more additional food ingredients to produce fiber-containing compositions. Methods for the production and use of compositions comprising the soluble -glucan fiber are also provided.

The present disclosure features compositions and methods for the treatment of actinomycetia (e.g., corynebacteriales) infections, e.g., caused by mycobacterial cells, residing within a host cell (e.g., a mammalian cell, e.g., immune cell, e.g., macrophage or dendritic cell). The compositions and methods include delivering a mixture of lytic enzymes to specifically target the cell envelope of a group of related bacterial species in the intracellular compartment (endosome, phagosome, lysosome, or cytosol) in which above mentioned orders of bacteria infect, grow, hide, and go quiescent.

The present disclosure features compositions and methods for the treatment of actinomycetia (e.g., corynebacteriales) infections, e.g., caused by mycobacterial cells. In one aspect, the disclosure features a composition containing unencapsulated proteins that include one or more of (a) a Lysin A; (b) a Lysin B; (c) an isoamylase; and (d) an -amylase. The composition optionally further includes a supramolecular structure including one or more (a) a Lysin A; (b) a Lysin B; (c) an isoamylase; and (d) an -amylase. In another aspect, the invention features a method of treating a bacterial infection in a subject by administering a composition of unencapsulated lytic proteins as described herein to the subject in an amount and for a duration sufficient to treat the bacterial infection, optionally in combination with encapsulated lytic proteins. The encapsulated lytic proteins and the unencapsulated lytic proteins can be in the same or different composition and can be administered simultaneously or sequentially.