The present invention relates to mannanase variants with improved stability. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Disclosed are beneficial uses of Dendrobium polysaccharides, and compositions comprising them and vancomycin-sensitive bacteria for intestinal disorders. The present disclosure relates to a method of treating an intestinal disorders, such as an inflammatory bowel disease, comprising administering Dendrobium polysaccharide to an individual in need thereof. The present disclosure also relates to a method of treating an intestinal disease comprising administering vancomycin-sensitive bacteria to an individual in need thereof, wherein said individual has taken or is about to take Dendrobium polysaccharide.