A method of using lactase for generating galactooligosaccharide as well as the preparation and an application of the lactase are provided. Lactase (BglD305 derived from Bacillus circulans B2301 and BglD derived from Bacillus circulans ATCC 31382) molecules from two sources are taken as the basis for molecular evolution, so as to obtain new lactase enzyme molecules with high galactooligosaccharide synthesis efficiency and good expression performance. The high-producing strain lactase is further constructed, the lactase can be efficiently synthesized during the submerged fermentation, and the enzyme molecule is secreted into the culture medium, the high-activity enzyme preparation is directly prepared from the fermentation supernatant, and the lactase expression level can achieve 2208 U/mL. As the result, the fermentation manufacturing cost of lactase is reduced, the fermentation manufacturing process is simplified, and the quality of the lactase preparation is improved.

Articles and methods related to the administration of biomolecules to subjects using metal-organic frameworks are generally described. In some embodiments, an article is described, the article comprising a substrate comprising an adhesive layer, a support layer disposed on at least a portion of the substrate, and an active material disposed on and/or in at least a portion of the support layer, wherein the active material comprises a composite comprising a metal-organic framework and a biomolecule. In certain embodiments, a method of treating a subject with a biomolecule is described, the method comprising administering an article to a subject.

Disclosed is an enzyme composition for regulating sugar metabolism which can regulate the absorption of glucose into the body by converting the carbohydrates in food to a form of sugar that is not absorbed in the stomach and the like before being decomposed in the small intestine into glucose by the activity of various enzymes such as maltase, sucrase, or lactase and the like and absorbed, wherein the enzyme composition includes: one or more enzymes selected from the group consisting of glucoamylase, sucrase and lactase; glucose oxidase; and transglucosidase.

A lactase bulk powder and a lactase preparation both have improved storage stability. The stability of a lactase bulk powder is improved by regulating the acceptable amount of glucose and/or galactose, generated during manufacturing the lactase bulk powder, within a preset range. The lactase bulk powder includes lactase, galactose and/or glucose. The total amount of galactose and glucose is more than 0 μmol and not more than 50 μmol per 100,000 U of lactase. The lactase preparation has the lactase bulk powder as an active ingredient.

Raw materials of a fermented milk include partially degreased raw milk, 4-10% w/w of saccharide based on the mass of the raw milk, 0.06-0.15% w/w of lactase based on the mass of the raw milk, 0.03-0.18% w/w of a stabilizer based on the mass of the raw milk, and 20-50 dcu of fermenting bacteria added per 100 kg of the raw milk; and the fermenting bacteria is one or more selected from the group consisting of Lactobacillus paracasei, Lactobacillus plantarum, Lactobacillus acidophilus, Bifidobacterium lactis and Streptococcus thermophilus, and preferably one or more selected from the group consisting of Lactobacillus plantarum with a preservation number of CGMCC No. 19748, Lactobacillus acidophilus with a preservation number of CGMCC No. 1084 and Streptococcus thermophilus. According to the fermented milk and a preparation method thereof, the prepared fermented milk has a more stable state during the shelf life.

The present invention provides a lactase-containing double microcapsule which includes; lactase provided as a core material; and a primary coating material and a secondary coating material, which are sequentially coated on the core material to be formulated, a method for preparing the lactase-containing double microcapsule, and a dairy product including the lactase-containing double microcapsule as a use of the lactase-containing double microcapsule.

Disclosed are compositions and methods useful for improving blood cholesterol profiles in a mammal, particularly for improving metabolism of cholesterol, for reducing the levels of low-density lipoprotein cholesterol (LDL-C) in the blood, increasing the levels of high-density lipoprotein (HDL-C) in the blood, for improving weight loss in a mammal, and/or for enhancing overall cardiovascular health in a mammal. Methods can involve identifying a mammal in need of lowering of blood LDL-C and/or triglyceride concentrations, and administering to said mammal a specific formulation consisting of a blend of probiotics; specifically, Bifidobacterium infantis, Bifidobacterium bifidum, Lactobacillus acidophilus, Lactobacillus salivarius, Lactobacillus plantarum, Lactobacillus rhamnosus, Bifidobacterium longum, Lactobacillus casei, Lactobacillus paracasei, in combination with a blend of digestive enzymes; specifically, amylase, glucoamylase, lipase, bromelain, maltase, lactase, hemicellulase, xylanase, papain, and invertase. Preferably, the aforementioned probiotics and digestive enzymes are combined into capsules and administered to said mammal three times daily to achieve said lowering of LDL-C and triglyceride concentrations in blood.

The present invention discloses the potential of probiotic bacteria Bacillus coagulans individually or in combination with multi-enzyme complex for reducing gluten content in foods rich in gluten and for the increased utilization of gluten. The invention further discloses a method for the management of gluten intolerance using composition comprising Bacillus coagulans and multi-enzyme complex in mammals.

The present invention relates to compositions, particularly liquid compositions, comprising polypeptides having beta-galactosidase activity, methods of making said compositions, and uses of the compositions for making e.g. dairy products. The polypeptides having beta-galactosidase activity are modified by glycation of lysine and/or arginine residues by incubating the enzyme in the presence of reducing sugars, optionally combined with a heat treatment. Thereby, transgalactosylating activity is increased.

The present invention relates to compositions, particularly liquid compositions, comprising polypeptides having beta-galactosidase activity, methods of making said compositions, and uses of the compositions for making e.g. dairy products. The polypeptides having beta-galactosidase activity are modified by glycation of lysine and/or arginine residues by incubating the enzyme in the presence of reducing sugars, optionally combined with a heat treatment. Thereby, transgalactosylating activity is increased.