Some aspects of this disclosure provide compositions, strategies, systems, reagents, methods, and kits that are useful for the targeted editing of nucleic acids, including editing a single site within the genome of a cell or subject, e.g., within the human genome. In some embodiments, fusion proteins capable of inducing a cytosine (C) to guanine (G) change in a nucleic acid (e.g., genomic DNA) are provided. In some embodiments, fusion proteins of a nucleic acid programmable DNA binding protein (e.g., Cas9) and nucleic acid editing proteins or protein domains, e.g., deaminase domains, polymerase domains, and/or base excision enzymes are provided. In some embodiments, methods for targeted nucleic acid editing are provided. In some embodiments, reagents and kits for the generation of targeted nucleic acid editing proteins, e.g., fusion proteins of a nucleic acid programmable DNA binding protein (e.g., Cas9), and nucleic acid editing proteins or domains, are provided.

The present application relates to: a single base substitution protein; a composition comprising same; and a use thereof.

Protected DNA comprising a single-stranded DNA (ssDNA) cassette is provided. Further provided are uses of the protected DNA, methods for producing protected DNA, products generated in performing such methods (including intermediate and final products), and kits for use in such methods.

The present invention relates to improvements in the use of padlock probes whereby the ligation of the padlock probe may be controlled, and in particular to a method of detecting a target nucleic acid in a sample which comprises the use of a padlock probe complexed with a blocking oligonucleotide. The blocking oligonucleotide binds the target-binding regions of the padlock probe and holds them apart in a manner which prevents their ligation, until the padlock probe is in the vicinity of the target nucleic acid molecule, at which point the padlock probe is released from the blocking probe so that it can bind its target, thereby reducing background signal. Also provided is a kit comprising a padlock probe and blocking oligonucleotide, which can be used in the methods of the invention.

A chromatography-based multiplex nucleic acid detector includes an air supply device, a heating device, a polymerase chain reaction (PCR) pipeline, and a cover plate assembly. An air outlet of the air supply device is configured to communicate with a first end of an amplification reagent tube. The cover plate assembly is mounted on the heating device, where an observation window is formed on the cover plate assembly, a PCR pipeline installation position is formed on at least one of the cover plate assembly or the heating device, the PCR pipeline is configured to be mounted at the PCR pipeline installation position, the heating device is configured to heat the PCR pipeline, and a test strip is mounted at the observation window. A chromatography-based multiplex nucleic acid detection test strip and a chromatography-based multiplex nucleic acid detection method are further provided.