The present invention provides host cells and methods for making mogrol glycosides, including Mogroside V (Mog. V), Mogroside VI (Mog. VI), Iso-Mogroside V (Isomog. V), and glycosylation products that are minor products in Siraitia grosvenorii. The invention provides engineered enzymes and engineered host cells for producing mogrol glycosylation products, such as Mog. V, Mog. VI, and Isomog. V, at high purity and/or yield. The present technology further provides methods of making products containing mogrol glycosides, such as Mog. V, Mog. VI, and Isomog. V, including food products, beverages, oral care products, sweeteners, and flavoring products.

A fusion protein and an immobilized enzyme including the fusion protein are provided. The fusion protein includes an epoxide hydrolase active domain and a chitin protein binding domain. Efficient immobilization of epoxide hydrolase is achieved through the specific affinity between chitin and the fusion protein, and ethylhexyl glycidyl ether is used as a substrate to efficiently produce ethylhexylglycerin with the immobilized enzyme. The immobilization method offers advantages such as low cost, high enzyme immobilization efficiency, minimal enzyme activity loss, and strong specificity, fundamentally solving issues like poor recyclability and low stability of free enzymes, as well as low repeatability in traditional whole-cell immobilization methods. It resolves issues such as deep color and protein residues in downstream separation and purification.