The present invention relates to compositions and methods comprising a chimeric antigen receptor (CAR) capable of binding fibroblast activation protein (FAP) for use in treating diseases, disorders or conditions associated with the expression of FAP on canine, mouse, or human tumor-associated cells.

This invention relates to the biosynthetic production of macrocyclic molecules from linear precursor peptides that contain highly truncated C terminal recognition sequences of 10 or fewer residues using prolyl oligopeptidase (POP) macrocyclases. This may be useful for example in the biosynthetic production of macrocyclic molecules. Methods and kits for the production of macrocyclic molecules, as well as libraries and methods of screening, are provided.

The present invention relates to a composition comprising hydrogels from functionalized hyaluronic acid derivatives, said hydrogels loaded with exogenous enzymes selected in the group consisting of prolyl endopeptidase (PEP) and endoprotease (EP) intended for the oral treatment of celiac disease. Specifically, this invention concerns a one-pot methodology useful to prepare methacrylic derivatives of hyaluronic acid, through the formation of a specific active group on hydroxyl groups of hyaluronic acid, the subsequent substitution of the inserted active group with ethylenediamine and finally, the reaction with methacrylic anhydride. The obtained methacrylic hyaluronic acid derivatives are used to prepare hydrogels through irradiation and loaded with exogenous enzymes selected in the group consisting of prolyl endopeptidase (PEP) and endoprotease (EP). The ability of prepared hydrogels to allow the enzyme release, as active form in simulated gastrointestinal fluids is proved.

An embodiment is a method of mitigating or treating Gastroesophageal Reflux Disease (GERD) caused by a neurological disorder that includes administering an effective amount of a medicament that includes a supernatant resulting from culturing Lactobacillus bulgaricus B-30892 bacteria to a human to mitigate or treat GERD caused by a neurological disorder.

The present invention relates to a process for preparing a yeast hydrolysate comprising less than 100 ppm gluten and at least 1 ppm gluten based on salt-free yeast dry matter.

The invention relates generally to the field of microbiology and molecular biology. Provided herein is an expression construct for producing a recombinant protein. The subject specification discloses an expression construct for producing a prolyl endopeptidase protein in lactic acid bacteria and methods of treatment comprising use of such a prolyl endopeptidase.

The present disclosure relates to methods of expressing recombinant neprosin polypeptide, host cells and expression vectors for expressing the recombinant neprosin polypeptide, and methods of using the expressed neprosin polypeptide for therapeutic and other purposes.

Fluorescence resonance energy transfer (FRET) constructs comprising donor and acceptor fluorophore moieties, and a peptide linking the two, which is a substrate of the endopeptidase fibroblast activation protein (FAP). Also provided are isolated nucleic acids expressing the construct, cell lines comprising the nucleic acids, and kits comprising the construct. Further provided are methods of detecting FAP using the construct via FRET.

Systems and methods are disclosed for the treatment of cancer. Specifically, techniques are disclosed for treating cancer through the administration of genetically modified immune cells that overexpress fibroblast activation protein. In other embodiments, the techniques include the treatment of cancer through the administration of fibroblast activation protein inhibitors to the tumor site.

Disclosed herein are fibroblast activation protein (FAP)-specific binding polypeptides. These binding polypeptides may be incorporated into chimeric antigen receptors (CARs). Also disclosed herein are methods of using these binding polypeptides and/or CARs for the treatment of, for example, a cancer.