The disclosure relates to detergents and cleaning agents containing a combination of protease and amylase, the amino acid sequence of which has been modified specifically for use in detergents and cleaning agents. The disclosure further relates to methods and uses of said detergents and cleaning agents.

A process of recovering oil, comprising (a) converting a starch-containing material into dextrins with an alpha-amylase; (b) saccharifying the dextrins using a carbohydrate source generating enzyme to form a sugar; (c) fermenting the sugar in a fermentation medium into a fermentation product using a fermenting organism; (d) recovering the fermentation product to form a whole stillage; (e) separating the whole stillage into thin stillage and wet cake; (e′) optionally concentrating the thin stillage into syrup; (f) recovering oil from the thin stillage and/or optionally the syrup, wherein a protease and a phospholipase are present and/or added during steps (a) to (c). Use of a protease and a phospholipase for increasing oil recovery yields from thin stillage and/or syrup in a fermentation product production process.

The present invention relates to compositions and a process in the field of self-cleaning system using digestive proteins. One composition includes a substrate, a digestive protein capable of decomposing a stain molecule, and a link moiety bound to both said digestive protein and said substrate. An alternative composition includes a digestive protein capable of decomposing a stain molecule and a coating substrate wherein said digestive protein may be dispersed in said coating substrate. The process claim includes binding a substrate to a surface and forming a linker moiety between a digestive protein and said substrate.

The present invention relates to a textile washing agent, in particular a liquid textile washing agent, comprising a) at least one protease, wherein the protease has proteolytic activity and comprises an amino acid sequence which has at least 70% sequence identity with the amino acid sequence given in SEQ ID NO:1 over its entire length and, in each case based on the numbering according to SEQ ID NO:1, has (i) at the positions that correspond to positions 9, 130, 133, 144, 217, 252 and 271, amino acid substitutions, in particular the amino acid substitutions 9T, 130D/V, 133A, 144K, 217M, 252T and 271E, and (ii) at at least one, preferably at least two, of the positions that correspond to positions 6, 89, 131, 166, 189, 211 or 224, at least one additional amino acid substitution, in particular selected from 6W/F, 89A/G, 131H/Y/F, 166M/L/I, 189T/L/I, 211N/Q and 224A/G, more preferably selected from 6W, 89A, 131H, 166M, 189T, 211N and 224A, and b) at least one washing agent ingredient, preferably in an amount of from 0.01 to 99.9 wt.%, wherein the textile washing agent has a pH of from approximately 9 to approximately 12, measured in a 1 wt.% solution in deionized water at 20° C. The present invention also relates to corresponding methods for cleaning textiles, in which methods agents according to the invention are used, and to uses of agents according to the invention for removing protease-sensitive stains from textiles.

The present invention relates to compositions comprising protease variants suitable for use in cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions.

The present invention relates to subtilase variants suitable for use in, e.g., cleaning or detergent compositions, such as laundry detergent compositions and dish wash compositions, including automatic dish wash compositions. The present invention also relates to isolated DNA sequences encoding the variants, expression vectors, host cells, and methods for producing and using the variants of the invention.

An agent and a method for cleaning textiles or hard surfaces are provided herein. In one embodiment, the agent includes a first protease and at least one second protease. The first protease Includes an amino acid sequence that is at least about 80% identical to the amino acid sequence indicated in SEQ ID NO.1. The second protease is any protease that is different from the first protease. In another embodiment, the method includes activating proteolytically the first protease and the at least one second protease.

The invention relates to moderate pH and optionally low conductivity powder detergent compositions comprising a protease.

The present invention relates to protease variants and methods for obtaining protease variants. The present invention also relates to polynucleotides encoding the variants; nucleic acid constructs, vectors, and host cells comprising the polynucleotides; and methods of using the variants.

Provided is a method for collecting an objective substance such as vanillin from a fermentation broth. Upon collecting an objective substance from a fermentation broth containing the objective substance by solvent extraction using an organic solvent, emulsification during the solvent extraction can be prevented by treating the fermentation broth with a protease and then subjecting it to the solvent extraction, or by carrying out the solvent extraction with an agitation power adjusted to a predetermined range, and thereby the objective substance can be collected from the fermentation broth.