The invention relates to the field of molecular biology, preparative biochemistry, biotechnology, and biopharmacology, namely to the creation of recombinant proteins of the family of wheat (Triticum aestivum) cysteine proteases in soluble form, and preparations of the protein triticain-alpha consisting of a fragment of wheat triticain-alpha and methods for the production thereof. The invention can be used for research purposes to study the functioning of papain-like cysteine proteases, as well as in medicine for developing therapeutic enzyme preparations, and is a method of producing, in soluble form, recombinant functionally active variants of wheat (Triticum aestivum) cysteine proteases, including the engineering of plasmid DNA for cloning in expression systems of E. coli and P. Pastoris. By transforming cells of E. coli of the strain Rossetta gami B (DE3) and cells of P. pastoris of the strain GS11.5 by means of plasmid DNA pET15-6HIS-tritcain-α-GM, pET15-triticain-α-GM-6HIS, and pPIC9-triticain-α-GM respectively, truncated producing strains of wheat triticain-alpha are obtained, with subsequent culturing of host cells, separation of expressing protein, and purification by chromatographic methods. The invention allows variants of a biologically active fragment of wheat protease to be produced in soluble form in bacteria and yeast expression systems and allows the preparation triticain-alpha to be produced with a high, stable output, purity level and functional activity.

The invention provides a peptide for complexing zinc ion, complex thereof and use therefor. The amino acid composition and sequence of the peptide for complexing zinc ion are Lys-Tyr-Lys-Arg-Gln-Arg-Trp (SEQ ID NO: 1). The peptide for complexing is derived from soybean or peanut, is an inherent component of foods, and has a super strong complexing effect with zinc ions.

Current methods for detection of microbial contaminants on surfaces use swabbing/wiping to extract microbes for analysis. This removes easily transferable microbes but fails to extract microbes living in biofilms, which reduces sensitivity and may mask the true degree of contamination. The current disclosure provides an enzyme cocktail that disrupts the biofilm and improves the extraction of live microbes for analysis. Applicant's enzyme system is particularly useful for the application to a variety of surfaces, but particularly on a variety of food processing surfaces. Utilization of Applicant's enzyme cocktail makes possible the extraction of a representative sample of live microorganisms present on a surface, including film forming microorganisms, without affecting non-film forming microorganisms also present on a surface.

The present invention relates to high-efficiency encapsulation of hydrophilic substances in the hydrophilic space of unilamellar liposomes. High-efficiency encapsulation is achieved by the use of a polyhydric alcohol selected from propylene glycol or glycerine for dissolving the hydrophobic compounds forming the lipid bilayer of the liposomes. The invention provides unilamellar liposomes (UL) as well as a method for preparing same by way of low temperature extrusion. The invention also relates to use of these UL in the manufacturing of a medicament, a cosmetic product, a food additive or a disinfectant.

The present invention relates to high-efficiency encapsulation of hydrophilic substances in the hydrophilic space of unilamellar liposomes. High-efficiency encapsulation is achieved by the use of a polyhydric alcohol selected from propylene glycol or glycerine for dissolving the hydrophobic compounds forming the lipid bilayer of the liposomes. The invention provides unilamellar liposomes (UL) as well as a method for preparing same by way of low temperature extrusion. The invention also relates to use of these UL in the manufacturing of a medicament, a cosmetic product, a food additive or a disinfectant.

The invention relates to the field of molecular biology, preparative biochemistry, biotechnology, and biopharmacology, namely to the creation of recombinant proteins of the family of wheat (Triticum aestivum) cysteine proteases in soluble form, and preparations of the protein triticain-alpha consisting of a fragment of wheat triticain-alpha and methods for the production thereof. The invention can be used for research purposes to study the functioning of papain-like cysteine proteases, as well as in medicine for developing therapeutic enzyme preparations, and is a method of producing, in soluble form, recombinant functionally active variants of wheat (Triticum aestivum) cysteine proteases, including the engineering of plasmid DNA for cloning in expression systems of E. coli and P. Pastoris. By transforming cells of E. coli of the strain Rossetta gami B (DE3) and cells of P. pastoris of the strain GS115 by plasmid DNA pET15-6HIS-tritcain-α-GM, pET15-triticain-α-GM-6HIS, and pPIC9-triticain-α-GM respectively, truncated producing strains of wheat triticain-alpha are obtained, with subsequent culturing of host cells, separation of expressing protein, and purification by chromatographic methods. The invention allows variants of a biologically active fragment of wheat protease to be produced in soluble form in bacteria and yeast expression systems and allows the preparation triticain-alpha to be produced with a high, stable output, purity level and functional activity.

A method for preparation of low molecular weight porcine lympho-reticular polypeptides. The method comprises the enzymatic hydrolysis of a source of protein, wherein the source of protein comprises a blend of porcine liver and porcine spleen, with a first enzyme having proteolytic activity and a second enzyme having amylase activity.

The present invention relates to a method for isolating and culturing neural stem cells with high efficiency, which may shorten the time for isolation and culture by simplifying a method for isolating and culturing neural stem cells and may increase the acquisition yield of neural stem cells. The present invention provides a method for isolating and culturing neural stem cells with high efficiency, comprising the steps of adding brain tissue into an enzyme solution so as to subject the brain tissue to enzyme treatment; physically isolating cell clumps from the enzyme treated brain tissue by dividing the cell clumps according to size and removing impurities; and inoculating the cell clumps on a culture dish so as to subculture.

The present invention provides a novel proteolytic enzyme composition more particularly an orally administered proteolytic enzyme composition comprising of one or more acid proteases, one or more alkaline protease and one or more plant proteases. More particularly, the composition comprises of microbial (fungal, bacterial or other microbes) protease enzymes, proteases from plant and animals proteases thereof.

This invention provides a composition and a method for increasing biogenic methane gas production from subterranean coal deposits. The composition and method provides a nutrient protein hydrolysate that contains less than 0.5 percent of the amino acid L-tyrosine, which is most effective in significantly increasing methane gas released from subterranean coal deposits.