The present invention provides a method of remediating contaminated soil with heavy metal using a microorganism of Sporosarcina sp. Contaminated soil is inoculated with the Sporosarcina sp. KM-01 (Sporosarcina pasteurii KM-01) strain includes a base sequence of SEQ ID NO. 1, the Sporosarcina sp. KM-07 (Sporosarcina pasteurii KM-07) strain includes a base sequence of SEQ ID NO. 2, and the Sporosarcina sp. KM-12 (Sporosarcina pasteurii KM-12) strain includes a base sequence of SEQ ID NO. 3. The strains are capable of producing urease.

Provided are a potentiometric biosensor (100) and a method for detecting urea concentration in a sample. The potentiometric biosensor (100) comprises an indication electrode (23) and a reference electrode (24). A second reaction reagent on the indication electrode (23) comprises a urease but does not comprise an electron transporter. A first reaction reagent on the reference electrode (24) comprises an electron transporter but does not comprise a urease. In the case where no external voltage is applied, after a blood sample has been added, the urease catalyzes the reaction of urea in the sample, thereby causing a potential difference between the indication electrode (23) and the reference electrode (24). Furthermore, the potential difference between the indication electrode (23) and the reference electrode (24) is in a linear relationship with the urea concentration in the sample, and the urea concentration in the sample can be calculated according to such linear relationship.

Provided are a method and an apparatus for producing urea-free water that can be used when analyzing urea in sample water, and that can efficiently produce urea-free water in which the urea concentration is reduced to at least a level that does not affect the analysis. The method for producing urea-free water includes adding urease to urea-containing water, thereby decomposing urea, to obtain treated water containing urease, and passing treated water through separation membrane, thereby removing the urease, to obtain urea-free water.

The present disclosure relates to method of depositing a coating over a substrate, The present disclosure also relates to a resin composition and a polymerized coating disposed over a substrate.

Disclosed are methods of extracting an enzyme, comprising soaking a seed in an aqueous solution, homogenizing the seed to produce a homogenized suspension, and filtering the homogenized suspension to produce a crude extract, wherein the crude extract comprises urease and non-urease proteins.

Described herein are novel construction materials and construction material compositions, processes, and equipment for manufacturing press-formed biocement and bioconcrete products and construction materials. In some embodiments, the methods include combining aggregate particles with a first measured dose of at least one biological organism or enzyme and a first measured dose of cementation reagents in a first mixer, mixing the contents of the first mixer and reacting the first measured dose of the cementation reagents in the presence of the first measured dose of the at least one biological organism or enzyme to form a biocement which binds to the surfaces of the aggregate particles, thereby increasing the size of the particles to yield a biocement coated aggregate.

Disclosed are methods of extracting an enzyme, comprising soaking a seed in an aqueous solution, homogenizing the seed to produce a homogenized suspension, and filtering the homogenized suspension to produce a crude extract, wherein the crude extract comprises urease and non-urease proteins.

This invention relates to a device that can be used is used to detect organophosphates and carbamate on surfaces including food, clothing (including as wearable pesticide detectors) and machinery.