Provided relates to the field of genetic engineering. In particular, a method for base editing in plant is provided. More specifically, a highly efficient A to G base editing method for a target sequence in a genome of a plant (e.g., a crop plant) by a guide RNA-directed CRISPR-adenine deaminase fusion protein and the plant and their progeny produced by the method are provided.

Described herein are DNA-editing complexes, particularly DNA-editing complexes that specifically alter a single base pair in target DNA sequence as well as methods of making and using these DNA-editing complexes.

Described herein are methods of modifying or editing a target nucleic acid such as methods that edit cytosine to thymine and adenine to guanine and/or methods that edit cytosine to thymine, adenine, or guanine. Compositions and systems for modifying or editing a target nucleic acid are also described. Methods, compositions and systems described herein may be used for generating allelic diversity.

This invention relates to Type V CRISPR-Cas effector proteins, deaminases, and fusion and recruiting nucleic acid constructs thereof. The invention further relates methods of targeted nucleic acid modification utilizing the same.

The invention relates to peptide linkers and fusion proteins comprising linkers designed for optimizing the activity of the proteins comprised therein, and methods for using the same. The invention further relates to newly designed Cas12a-based adenine base editors.

Provided relates to the field of genetic engineering. In particular, a method for base editing in plant is provided. More specifically, a highly efficient A to G base editing method for a target sequence in a genome of a plant (e.g., a crop plant) by a guide RNA-directed CRISPR-adenine deaminase fusion protein and the plant and their progeny produced by the method are provided.

Described herein are DNA-editing complexes, particularly DNA-editing complexes that specifically alter a single base pair in target DNA sequence as well as methods of making and using these DNA-editing complexes.

The present invention provides genomic engineering methods that utilize base editors to perform targeted gene knockins and gene knockouts with high efficiency. Cells that have been genetically modified according to these methods are also provided, as are methods of using the modified cells in a treatment for cancer.

The invention relates to methods for clustered regularly interspaced short palindromic repeat (CRISPR)-mediated A to G and/or C to T editing of the guide-complementary strand of a double stranded target DNA. The invention further relates to a nucleotide molecule encoding said CRISPR-mediated base editing system, to an expression vector comprising the nucleotide molecule, and to a cell comprising the expression vector. The invention further relates to an isolated Cas nuclease that allows A to G and/or C to T editing of the complementary strand of a double stranded target nucleic acid.

Disclosed herein are methodologies and kits for dynamic targeted hypermutation that harness the enzymatic activity of a polynucleic acid-binding protein fused to a nucleobase-editing enzyme to specifically target mutations across a region of interest. These methodologies and kits facilitate the rapid creation of diverse DNA libraries in vivo or in vitro.