Provided herein are substantially non-luminescent peptide/polypeptide tags that are inserted internally within a protein of interest or between N-terminal and C-terminal peptides/polypeptides. Interaction of the internally-inserted tag with a complement polypeptide/peptide that is also substantially non-luminescent results in the formation a bioluminescent reporter complex.

Provided are a dehalogenase variant, a polynucleotide encoding the dehalogenase variant, a recombinant microorganism including a genetic modification that increases dehalogenase activity, a composition including the recombinant microorganism, and a method of reducing a concentration of fluorinated methane using the recombinant microorganism.

Provided is a microorganism including a gene encoding a protein having a dehalogenase activity, a composition for using in reducing a concentration of fluorinated methane in a sample, the composition including the microorganism including the gene encoding the protein having the dehalogenase activity, and a method of reducing the concentration of fluorinated methane in the sample.

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate and has at least two amino acid substitutions relative to the wild-type hydrolase. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

Nucleic acid-guided ordered protein assembly (NOPA) arrays and methods for their generation and related applications are disclosed herein.

A mutant hydrolase optionally fused to a protein of interest is provided. The mutant hydrolase is capable of forming a bond with a substrate for the corresponding nonmutant (wild-type) hydrolase which is more stable than the bond formed between the wild-type hydrolase and the substrate. Substrates for hydrolases comprising one or more functional groups are also provided, as well as methods of using the mutant hydrolase and the substrates of the invention. Also provided is a fusion protein capable of forming a stable bond with a substrate and cells which express the fusion protein.

The present invention relates to a kit for analyzing interactomes, and a method for analyzing interactomes through photo-crosslinking by using the kit. According to the kit for analyzing interactomes and the method for analyzing interactomes by using same, in one aspect of the present invention, interactomes are linked through a covalent bond one by one so that analysis errors can be reduced. In addition, according to the present invention, interactomes in living cells can be analyzed and interactomes in the nucleus of cells can be analyzed. Furthermore, according to the present invention, the formation time point of interactomes can be regulated so that target proteins and interactomes can be analyzed in a specific situation or time point.

The invention relates to a modular polypeptide comprising a first partial effector sequence comprising a first part of a circular permutated halotag protein connected to a sensor module sequence, which is connected to a second part of a circular permutated halotag protein. The sensor module is a single polypeptide or a polypeptide pair capable of undergoing conformational change from a first confirmation to a second confirmation depending on the presence or concentration of an analyte compound. The modular peptide is catalytically active in response to an environmental stimulus or in response to the sensor pair interacting. The invention further relates to nucleic acid sequences encoding the modular polypeptide, and to kits comprising same.

Embodiments of the present disclosure relate to methods, compositions, and systems for proximity-based, photoactivated labeling of molecules. Molecules may be labeled via activation of a ligated photocatalyst capable of transmitting energy to a proximal biomolecular labeling agent. Depending on the activated half-life and diffusion coefficient of the labeling agent, molecules within a particular vicinity of the ligated photocatalyst may be labeled but molecules outside the vicinity will not be labeled.