Described herein is a novel enzymatic pathway and acid tolerant Schizosaccharomyces pombe strain exhibiting the same, for producing 3-hydroxypropionic acid by fermentation wherein the S. pombe strain includes nucleotide sequences that encode, and which are operably linked to promoters to express, non-native enzymes that exhibit an oxaloacetate decarboxylase (ODC) activity that converts oxaloacetate to 3-oxopropionate and a 3-oxopropionate reductase (OPR) activity that converts the 3-opropionate to 3-hydroxypropianate. ODC and OPR enzymes are not known to exist in nature, nor is an enzymatic path for making 3-hydroxypropionic acid but particular enzymes exhibiting the requisite activities are herein identified by sequence. A further enhancement is to also overexpress an enzyme that has at least one of a pyruvate carboxylase activity, a phosphoenolpyruvate carboxy kinase activity, and a phosphoenolpyruvate carboxylase activity, which increases the level of oxaloacetate in the cell leading to greater 3-HP production.