Ribulose-1,5-bisphosphate oxygenase (RuBisCO) protein fibers and a method of producing them are disclosed herein. The method of producing one or more RuBisCO protein fibers including obtaining RuBisCO, tbr example from tobacco, combining the RuBisCO with one or more plasticizers, heating the combination of the RuBisCO and the one or more plasticizers up to about 140 degrees C., filtering the heated combination through an about 20 ?m filter, and passing the filtered combination through an orifice to produce one or more RuBisCO protein fibers.

The disclosure describes methods for the purification of protein-enriched extracts to provide concentrates and isolates and methods for incorporation of such materials into products. The purification methods are adapted for removal of nicotine and may have other benefits, e.g., lightening the color of the protein-enriched extracts. The methods generally include treatment with peracetic acid or hydrogen peroxide and filtrations. A protein composition in the form of a concentrate or isolate is provided, the protein composition including RuBisCO, F2 fraction proteins, or combination thereof extracted from a plant of the Nicotiana species, wherein the protein composition is characterized by relatively low nicotine content.

Disclosed are plants including a cyanobacterial ribulose-1,5,-bisphosphate carboxylase/oxygenase (Rubisco) which can assemble and fix carbon without an interacting protein.

The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism.

Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.

Materials and methods for extracting and purifying proteins are provided. For example, the materials and methods provided herein can be used for extracting and purifying proteins that denature at low temperature.

The present disclosure relates to a dry chloroplast composition comprising chloroplasts isolated from Fabaceae family plants. The composition has a moisture content of less than about 8%. The composition has at least one ratio chosen from: a ratio of chlorophyll/fructose 1,6-biphosphatase (FBPP) of about 50 to about 130, the ratio of chlorophyll/FBPP being

[00001]$\frac{\mathrm{chlorophyll}\mathrm{in}\left(\mathrm{mg}/g\right)}{\mathrm{FBPP}\mathrm{in}\left(\mathrm{intensity}/\mathrm{mg}\mathrm{powder}\right)}?10000000,$ The FBPP intensity being measured by immunoblot, a ratio of Rubisco/chlorophyll of about 25 to about 65, the ratio of Rubisco/chlorophyll being

[00002]$\left(\frac{Ru\mathrm{bisco}\mathrm{in}\mathrm{intensity}}{chloro\mathrm{phyll}\mathrm{in}\left(\mathrm{mg}/g\right)}\right)/1000,$ wherein the Rubisco intensity being measured by immunoblot, a ratio of Rubisco/chlorophyll of about 2.5 ng/mg to about 6.5 ng/mg, the ratio of Rubisco/chlorophyll being

[00003]$ ( R u bisco in ng / mg powder c h l o r o phyll in ( mg / g ) ) / 1000< RECOMBINANT MICRO-ORGANISM FOR USE IN METHOD WITH INCREASED PRODUCT YIELD 20180251773 · 2018-09-06 · Antonius Jeroen Adriaan Van Maris, Jacobus Thomas Pronk, Victor Gabriel GUADALUPE MEDINA, Hendrik Wouter WISSELINK The invention relates to a recombinant yeast cell, in particular a transgenic yeast cell, functionally expressing one or more recombinant, in particular heterologous, nucleic acid sequences encoding ribulose-1,5-biphosphate carboxylase oxygenase (Rubisco) and phosphoribulokinase (PRK). The invention further relates to the use of carbon dioxide as an electron acceptor in a recombinant chemotrophic micro-organism, in particular a eukaryotic micro-organism. Yeasts modified to use carbon dioxide 10066234 · 2018-09-04 · Institut National De La Recherche Agronomique, INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE, Centre National De La Recherche Scientifique · Denis Pompon, Frederic Paques, Julie Lesage, Stephane Guillouet, Florence Bonnot, Jillian Marc, Nathalie Gorret, Carine Bideaux, Christel Boutonnet The invention relates to yeast cells modified to express a functional type I RuBisCO enzyme, and a class II phosphoribulokinase. The expression of these enzymes recreates a Calvin cycle in said yeasts in order to enable the yeasts to use carbon dioxide. IMMOBILIZATION OF BIOMOLECULES BY SELF-ASSEMBLED NANOSTRUCTURES 20180185502 · 2018-07-05 · Jon Robert PARQUETTE, Sriram Satagopan, Yuan SUN, Fred Robert TABITA Disclosed are nanostructures such as carboxysomes that encapsulate RubisCO and carbonic anhydrase to provide a protected environment to maximize CO.sub.2 assimilation. Conditions are disclosed were RubisCO can be sequestered into a variety of self-assembling nanotubes. The encapsulated protein was enzymatically active and was clearly associated with the nanotubes and removed from solution based on a number of criteria. These nanostructures were also found to enhance the stability of RubisCO toward proteases and other environmental factors. These structures can be used in scalable CO.sub.2 conversions and other processes. $