This disclosure relates to recombinant microbes, systems, and methods useful in the production of psilocybin and dimethyltryptarme (DMT). The disclosure relates to modifications and optimizations to genes and enzymes directly involved in the psilocybin and DMT pathway for the production of psilocybin and DMT in a host cell. The disclosure also relates to modifications and optimizations to genes and enzymes in the host cell and medium for psilocybin and DMT production in E. coli. The present disclosure also relates to a semi-synthetic method of producing psilocybin.

The instant disclosure is in the field of biosciences, more particularly towards molecular and industrial biotechnology. The present disclosure relates to recombinant methanotrophic bacteria capable of synthesizing indigo from methane comprising a gene encoding enzyme for increasing concentration of indole and a gene encoding enzyme for converting the indole to indoxyl. The present disclosure also relates to a method of developing the recombinant methanotrophic bacteria, and a method of indigo biosynthesis by the recombinant methanotrophic bacteria in presence of a methane source.

Provided are a microorganism producing an L-amino acid or a precursor thereof, and a method of producing an L-amino acid or a precursor thereof using the microorganism.

Disclosed are processes and methods for production of pigment compounds, such as melanin, using engineered microbial systems and chemical modifications to intermediate compounds. One process involves producing a first intermediate (e.g., an amino acid like tyrosine) and a second intermediate (e.g., an enzyme like tyrosinase) within a microbial cell, exporting the intermediates to an extracellular medium, and enabling their reaction outside the cell to form the pigment compound. The microbial cell is genetically engineered to enhance production and export of intermediates, prevent reuptake, and optimize enzyme activity post-export. Additional intermediates or capping agents are introduced to modulate pigment properties, such as color, molecular weight, and solubility. The process may be applied to produce various pigments, including pheomelanin and violacein, and is scalable for industrial applications in textiles, cosmetics, and carbon sequestration. The system minimizes cellular toxicity, simplifies purification, and allows for tailored pigment production.