Sulfurihydrogenibium sp. carbonic anhydrase (SspCA) or mutants thereof catalyze a hydration reaction of CO.sub.2 into bicarbonate and hydrogen ions or a desorption reaction to produce a CO.sub.2 gas. Sulfurihydrogenibium sp. carbonic anhydrase (SspCA) having improved thermostability in the presence of carbonate ions as compared to in the absence of carbonate ions are useful in the capture of CO.sub.2 from a CO.sub.2-containing gas.

An aqueous solution of carbonic anhydrase and calcium chloride is contacted with a cementitious surface defining at least one opening or fissure in the presence of ambient carbon dioxide to thereby cause the calcium chloride and carbon dioxide to react, whereby calcium carbonate precipitate from the solution and seals the opening or fissure.

Recombinant DNA constructs comprise plastidic carbonic anhydrase coding sequences operably linked to polynucleotides encoding DGAT or transcription factors such as ODP1. These constructs can be used to increase oil content in plants and seeds to levels that are not achieved with DGAT alone. Methods of generating plants containing the constructs and for increasing oil content in the seeds of an oilseed plant are also disclosed.

This invention provides novel carbonic anhydrase (CAIX) nucleic acid and peptide sequences, as well as related methods and compositions, including anti-cancer immunogenic agent(s) (e.g. vaccines and chimeric molecules) that elicit an immune response specifically directed against cancer cells expressing a CAIX antigenic marker. The novel CAIX variant and related compositions are useful in a wide variety of treatment modalities including, but not limited to protein vaccination, DNA vaccination, and adoptive immunotherapy.

Methods for enzyme-enhanced CO.sub.2 capture include contacting a CO.sub.2-containing gas with an aqueous absorption solution at process conditionssuch as high temperature, high pH, and/or using carbonate-based solutionsin the presence of Thermovibrio ammonificans carbonic anhydrase (TACA) or functional derivative thereof for catalyzing the hydration reaction of CO.sub.2 into bicarbonate and hydrogen ions and/or catalyzing the desorption reaction to produce a CO.sub.2 gas. The TACA may be provided to flow with the solution to cycle through a CO.sub.2 capture system that includes an absorber and a stripper.

The present invention relates to a carbon dioxide conversion reactor and more particularly, to a carbon dioxide conversion reactor capable of converting carbon dioxide contained in flue gas into an aqueous bicarbonate solution that may be used in many applications; and at the same time, preventing back pressure from increasing due to supplied flue gas by allowing a conversion process to rapidly proceed, thereby significantly reducing the level of carbon dioxide contained in flue gas with high efficiency and high conversion speed, a series reactor for converting and capturing carbon dioxide including the carbon dioxide conversion reactor, and a process of converting and capturing carbon dioxide using the carbon dioxide conversion reactor.

Disclosed are nanostructures such as carboxysomes that encapsulate RubisCO and carbonic anhydrase to provide a protected environment to maximize CO.sub.2 assimilation. Conditions are disclosed were RubisCO can be sequestered into a variety of self-assembling nanotubes. The encapsulated protein was enzymatically active and was clearly associated with the nanotubes and removed from solution based on a number of criteria. These nanostructures were also found to enhance the stability of RubisCO toward proteases and other environmental factors. These structures can be used in scalable CO.sub.2 conversions and other processes.

The present disclosure is directed to the biosynthetic pathway for a nonribosomal peptide synthetase (NRPS) derived drug and analogs thereof. The invention provides polynucleotide sequences useful for heterologous expression in a convenient microbial host for the synthesis of the NRPS-derived drug, the polypeptides encoded by such polynucleotides, expression vectors comprising the polynucleotides, host cells comprising the polynucleotides or expression vectors, and kits comprising a host cell. Also provided is a method for the production of ET-743, the NRPS-derived drug.

This invention provides novel carbonic anhydrase (CAIX) nucleic acid and peptide sequences, as well as related methods and compositions, including anti-cancer immunogenic agent(s) (e.g. vaccines and chimeric molecules) that elicit an immune response specifically directed against cancer cells expressing a CAIX antigenic marker. The novel CAIX variant and related compositions are useful in a wide variety of treatment modalities including, but not limited to protein vaccination, DNA vaccination, and adoptive immunotherapy.

Use of Sulfurihydrogenibium sp. carbonic anhydrase (SspCA) or mutants thereof for catalyzing the hydration reaction of CO.sub.2 into bicarbonate and hydrogen ions or catalyzing the desorption reaction to produce a CO.sub.2 gas is provided.