The disclosure discloses an alginate lyase and application thereof, and belongs to the technical field of biology. The alginate lyase provided by the disclosure has high degradation activity, and the enzyme activity reaches 65 U/mg; the alginate lyase is stable in nature, and the enzyme activity remains 98% or higher of the initial enzyme activity after storage at 4? C. for 18 months; and the alginate lyase has high product specificity. The disclosure uses E. coli as a host to express the alginate lyase derived from V. natriegens, the obtained recombinant E. coli can produce the alginate lyase secreted extracellularly in a conventional LB medium without adding an induction substrate sodium alginate, so the downstream processing technology of protein is simplified, and the disclosure has great industrial application potential.

Provided herein are bacteriophages engineered to express an exopolysaccharide (EPS) depolymerase for treating bacterial infections. In some embodiments, the EPS depolymerase comprises alginate lyase. Also envisioned within the scope of the invention are compositions comprising one or more of the bacteriophages, methods for treating bacterial infections, and kits comprising the compositions described herein.

The present invention concerns genetically modified Mycoplasma bacteria that express gene products having an anti-biofilm activity against a biofilm formed by multiple bacteria such as Pseudomonas aeruginosa and Staphylococcus aureus. Further intended are pharmaceutical compositions comprising genetically modified Mycoplasma bacteria and their use as a medicament.

The invention provides a series of peptide signals which, when linked to a polypeptide of interest (POI), ensure that said polypeptide is secreted in high yields by a host cell such as Mycoplasma pneumoniae. The invention also provides fusion proteins tagged with said peptide signals, the nucleic acid sequences coding for them, host cells comprising said tagged fusion proteins and a variety of uses of the fusion proteins and the host cells.

Described herein are isolated recombinant proteins having lyase activity and nucleic acid sequences which code therefor; along with methods of expressing, isolating, purifying, and using same.

Described herein are isolated recombinant proteins having lyase activity and nucleic acid sequences which code therefor; along with methods of expressing, isolating, purifying, and using same.

Detergent compositions particularly for laundry, comprising alginate lyase enzyme, and a booster enzyme selected from one or more of (i) Pel-ase enzyme; (ii) Psl-ase enzyme; (iii) endo--1,3 (4)-glucanase enzyme; and (iv) mixtures thereof. Methods of treating fabrics by contacting the fabric with an aqueous wash liquor having the detergent composition therein. The compositions and methods are particularly for improving whiteness of a fabric, improved soil removal from a fabric, for malodour removal from a fabric, for anti-wrinkle benefits, collar and/or cuff cleaning, anti-redeposition benefits and/or for improved drying of a fabric.

A washing machine-cleaning composition comprising (a) at least two enzymes selected from (i) alginate lyase enzyme; (ii) Pel-ase enzyme; (iii) Psl-ase enzyme; (iv) -1,3-glucanase enzyme; and (v) -1,3 (4)-glucanase enzyme; and (b) a cleaning adjunct and a method of treating the interior of a washing machine and a method of degrading soils on an interior surface of an washing machine using such a composition.

Described herein are proteins having lyase activity and nucleic acid sequences which code therefor; along with methods of expressing, isolating, purifying, and using same.

The present disclose relates to a high-reactivity hydrogel particle and preparation method therefor and use thereof. The preparation method comprises the following steps: (1) performing polymerization reaction of double-bond modified polyethylene glycol, a modified natural polymer and an optional active functional monomer to obtain a gel; (2) smashing the gel to obtain a gel particle; and (3) performing enzymolysis reaction of the gel particle and an enzyme to obtain the hydrogel particle with reactivity. The double-bond modified polyethylene glycol has a double bond and a polyethylene glycol chain segment, the modified natural polymer has sulfydryl or a double bond, the active functional monomer is selected from a sulfydryl functionalized biological adhesive peptide or sulfydryl functionalized cholesterol, and the enzyme is an enzyme corresponding to the natural polymer. The obtained hydrogel particle has high reactivity and can be used for subsequent secondary crosslinking. The hydrogel particle also has good biocompatibility.