The present invention provides a method for purifying Chondroitinase ABC (ChABC). The present method includes using a heparin-immobilized affinity chromatography column, and through chromatography method obtaining a purified ChABC from a matrix containing said ChABC. The present method is capable of obtaining ChABC in high purity with the advantages of simplicity in preparation and high yield.

There is provided a package wherein a pharmaceutical composition containing a saccharide-degrading enzyme is contained in a container, whereby reduction in titer caused by a low amount of enzyme is suppressed. A package comprising a pharmaceutical composition and a container, wherein the pharmaceutical composition is a lyophilized preparation containing a saccharide-degrading enzyme as an active ingredient, and the container contains the pharmaceutical composition, the inner surface of the container comprising at least one material selected from the group consisting of fine ceramics, silicone resins and fluorine resins.

The present disclosure is directed to adeno -associated viral vector monoclonal antibody constructs and compositions thereof, methods of improving locomotor function after spinal cord injury, methods of treating neurodegenerative diseases.

The present invention provides a method for purifying Chondroitinase ABC (ChABC). The present method includes using a heparin-immobilized affinity chromatography column, and through chromatography method obtaining a purified ChABC from a matrix containing said ChABC. The present method is capable of obtaining ChABC in high purity with the advantages of simplicity in preparation and high yield.

The present disclosure is directed to adeno-associated viral vector monoclonal antibody constructs and compositions thereof, methods of improving locomotor function after spinal cord injury, methods of treating neurodegenerative diseases.

The present invention relates to the field of bio-production of chondroitin. There is a need in the art for chondroitin production methods allowing its highly efficient synthesis and secretion. The solution proposed in the present invention is the use of a recombinant cell, in particular a recombinant yeast, comprising many modifications as described in the present text. The present invention further proposes methods allowing the bio-production of chondroitin using the recombinant cell, in particular a recombinant yeast, of the invention.

This disclosure relates to compositions capable of use in the treatment of spinal cord injuries and related disorders of the central nervous system (CNS), and in particular, compositions including proteoglycan degrading molecules and compositions capable of blocking and/or over coming the activity of neuronal growth inhibitory molecules, as well as fusion proteins which includes a proteoglycan degrading domain and a domain capable of blocking and or over coming the activity of neuronal growth inhibitory molecules.

One aspect of the present invention relates to mutants of chondroitinase ABCI. Such chondroitinase ABCI mutants exhibit altered chondroitin lyase activity or increased resistance to inactivation from stressors including exposure to UV light or heat. Methods of using chondroitinase ABCI mutant enzymes are also provided.

Compositions are provided according to aspects of the present invention which include: a plurality of longitudinally extending fibers, each of the fibers having a longitudinal axis, wherein the longitudinal axis of each of a majority of the fibers is generally aligned; a plurality of stem cells capable of differentiation into a central or peripheral nervous system cell, a majority of the plurality of stem cells in contact with one or more of the plurality of longitudinally extending fibers; a biocompatible hydrogel, wherein the longitudinally extending fibers and stem cells are disposed in the matrix of a biocompatible hydrogel; a therapeutic amount of a neurotrophic factor disposed in the biocompatible hydrogel, wherein the neurotrophic factor is distributed as a gradient; a plurality of olfactory ensheathing cells disposed in the biocompatible hydrogel; and a therapeutic amount of a scar inhibitor disposed in the biocompatible hydrogel.

The present invention provides a therapeutic agent for disc herniation, which has extremely few adverse side effects, can achieve a prolonged pain-ameliorating effect when administered in only a single dose, and can exhibit a high therapeutic effect and high safety in clinical applications. The present invention relates to a therapeutic agent for disc herniation, which is characterized by containing chondroitinase ABC as an active ingredient and being administered in such a manner that the ingredient can be administered into a human disk in an amount of 1-8 units per disk.