A method includes forming a patterned substrate including a plurality of base pads, using a nano-imprint lithography process. A capture substance is attached to each of the plurality of base pads, optionally through a linker, the capture substance being adapted to promote capture of a target molecule.

The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing.

The present invention is concerned with compositions and methods for improving the rate of correct sample identification in indexed nucleic acid library preparations for multiplex next generation sequencing by blocking the 3′ ends of pooled indexed polynucleotides from multiple samples prior to amplification and sequencing.

Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.

Methods and compositions are disclosed relating to the localization of nucleic acids to arrays such as silane-free arrays, and of sequencing the nucleic acids localized thereby.

Described herein are in situ synthesized arrays and methods of making them, wherein array signal sensitivity and robustness is enhanced by carrying out conditioning steps and/or generating linkers during synthesis. An array comprises a surface with a collection of features, wherein the features comprise molecules or polymers attached to the surface. In certain embodiments of the invention, carrying out conditioning steps during array synthesis can yield arrays with improved signal. In other embodiments, linkers are synthesized on the array surface prior to synthesis of functional molecules, wherein increasing linker length can correspond to an improvement in the signal generated by the array.

Described herein are in situ synthesized arrays and methods of making them, wherein array signal sensitivity and robustness is enhanced by carrying out conditioning steps and/or generating linkers during synthesis. An array comprises a surface with a collection of features, wherein the features comprise molecules or polymers attached to the surface. In certain embodiments of the invention, carrying out conditioning steps during array synthesis can yield arrays with improved signal. In other embodiments, linkers are synthesized on the array surface prior to synthesis of functional molecules, wherein increasing linker length can correspond to an improvement in the signal generated by the array.

Process for printing an adhesive pattern on a polymer brush extending at the surface of a support (1), forming a nanometric anti-fouling layer (2), the process comprising the following steps: placing the layer (2) in contact with a first aqueous solution (4) containing a benzophenone, then illuminating the layer with radiation (3) at a wavelength within the absorption spectrum of benzophenone, according to the pattern and according to a surface energy.

Process for printing an adhesive pattern on a polymer brush extending at the surface of a support (1), forming a nanometric anti-fouling layer (2), the process comprising the following steps: placing the layer (2) in contact with a first aqueous solution (4) containing a benzophenone, then illuminating the layer with radiation (3) at a wavelength within the absorption spectrum of benzophenone, according to the pattern and according to a surface energy.

The present disclosure relates to processes for inverting oligonucleotide probes in an in situ synthesized array. These processes can be used to reverse the orientation of probes with respect to the substrate from 3-bound to a substrate to 5-bound to another substrate. These processes can also be used to reduce or eliminate the presence of truncated probe sequences from an in situ synthesized array. These processes can preserve the original patterns of the synthesized oligonucleotide after the inversion. These process can be achieved via the formation of a hydrogel layer in-between a donor substrate and an acceptor substrate through a polymerization reaction forming the hydrogel layer.