A recombinant microorganism including a genetic modification that increases activity of nitric oxide reductase in the recombinant microorganism, a composition for reducing a concentration of nitric oxide in a sample, the composition including the recombinant microorganism, and a method of reducing a concentration of nitric oxide in a sample, are disclosed.

The invention provides a dynamic membrane reactor with function of nitrogen and phosphorus removal and an operation method thereof, and comprises a biological treatment system, a dynamic membrane loading system and an automatic system. The operation method comprises the following steps. (1) Before the formation of dynamic membrane, a porous filter for phosphorus removal is used as a cathode, a conductive precision filter screen is used as an anode, and aerobic denitrifying bacteria are inoculated into the dynamic membrane reactor under certain constant current density, hydraulic retention time and flux. (2) After the dynamic membrane is formed, the porous filter for phosphorus removal is used as the anode, the conductive precision filter screen is used as the cathode. And intermittent aeration is started at the anode under certain constant current density. (3) When the transmembrane pressure difference exceeds a certain range, hydraulic backwashing is performed under certain constant current density.

The present invention belongs to the technical field of sewage denitrification. Disclosed are a sewage treatment system and method of continuous flow step-feed short-cut denitrification-anaerobic ammonia oxidation coupled with denitrification. The system comprises a primary sedimentation tank, a bioreactor and a secondary sedimentation tank, which are connected in sequence, wherein the bioreactor comprises a pre-starvation zone, a first hypoxic zone, a first aerobic zone, a second hypoxic zone and a second aerobic zone: an agitator is arranged in each of the pre-starvation zone, the first hypoxic zone and the second hypoxic zone; an aeration device is arranged in both the first aerobic zone and the second aerobic zone; a polyurethane sponge filler is arranged in both the first hypoxic zone and the second hypoxic zone; and a nitrification liquid reflux pump is arranged between the first aerobic zone and the pre-starvation zone. By rationally utilizing a carbon source of raw sewage, the present invention develops a sewage denitrification system and a process control method which have the advantages of an efficient denitrification effect, a simple procedure flow, being convenient in terms of operation and maintenance, and being automated and intelligent.

A sewage treatment apparatus is provided for reducing nitrogen content in sewage fluid (e.g., after primary treatment). The apparatus uses vegetation to process the sewage fluid and reduce ammonia and organic nitrogen in the processed sewage fluid by uptake of the ammonia and organic nitrogen into the vegetation and by converting the residual ammonia and organic nitrogen into nitrites and nitrates. The apparatus also uses a feedback loop to combine the processed sewage fluid and the raw sewage fluid, such that nitrites and nitrates in the processed sewage fluid are reduced by interacting with carbonaceous waste in the raw sewage fluid.

An efficient and low-cost process for gravity thickening and fermentation of waste activated sludge withdrawn from the surface of an activated sludge aeration basin for use with treatment systems designed for “enhanced biological phosphorus removal” (EBPR). One or more reactor tanks are used for the process with the steps of: A fill cycle, in which the waste mixed liquor flows into the tank, followed by a settle cycle, in which the mixed liquor is allowed to settle for a period of time, followed by a decant cycle, in which the clear liquid is withdrawn. The withdrawal of a volume of the settling mixed liquor for discharge is then followed by a ferment period for the remaining settled mixed liquor solids and a transfer of the fermented mixed liquor solids back to the activated sludge liquid stream process.

Disclosed are a baffled integrated denitrifying and decarbonizing device with anaerobic bio-nests and a baffled integrated denitrifying and decarbonizing process with anaerobic bio-nests thereof. The wastewater with low carbon-nitrogen ratio first enters anaerobic chamber I, then enters anaerobic chamber II and chamber III to complete anaerobic decarbonization and denitrification. The chambers are provided with modified basalt fiber carrier media to enrich a large number of functional microorganisms, and improve the device in terms of anaerobic treatment efficiency. Fermentation liquid in chamber III then flows back to aerobic chamber IV to complete the nitrification process. Nitrified liquid enters chamber I and mixes with influent for further treatment, and effluent is finally discharged from chamber III. The clapboard and basalt fiber felt in chamber IV can retain and enrich autotrophic/heterotrophic nitrifying bacteria.

An apparatus for treating municipal sewage by anaerobic/aerobic/anoxic (AOA) [1] process via simultaneous endogenous partial [2] denitrification coupled with anammox in anoxic zone is disclosed. The apparatus mainly includes a raw water tank (1) for sewage, an AOA reactor (2) and a sedimentation tank (3), the sludge flows back from the bottom of the sedimentation tank (3) to the anoxic zone (2.4) and the anaerobic zone (2.2) respectively, and biofilm filler is added to the anoxic zone (2.4). The sewage enters the AOA reactor (2), and the intracellular carbon source is stored in the anaerobic zone (2.2) to remove the organic matter in the raw water. Then it enters the aerobic zone (2.3) for nitrification, and the generated nitrate-nitrogen enters the anoxic zone (2.4) for endogenous partial denitrification. The filler in the anoxic zone (2.4) uses the generated nitrite-nitrogen by endogenous partial denitrification and the remaining ammonia-nitrogen in the raw water to perform anammox reaction. The generated nitrate-nitrogen can be further removed by endogenous denitrification in the anoxic zone (2.4). Endogenous partial denitrification coupled with anammox is used for nitrogen removal in the anoxic zone (2.4), which can reduce the requirement of aeration in the aerobic zone (2.3) and the carbon sources in the anoxic zone (2.4), and suitable for low C/N ratio municipal sewage treatment. A method for treating municipal sewage by AOA process via endogenous partial denitrification coupled with anammox in anoxic zone is also provided.

A system for transferring marine life within an aquaculture facility including a plurality of segregated storage facilities each containing water for marine life, maintained within a predetermined temperature range and supported at independent ground levels. The storage facilities are successively disposed and structured to contain marine life at different stages of growth. A transfer assembly includes a path of fluid flow interconnecting successive ones of said plurality of storage facilities in fluid communication with one another, wherein at least a majority of a length of said path of fluid flow is disposed beneath the independent ground levels at a predetermined depth, which is sufficient to facilitate maintenance of the path of fluid flow within the predetermined temperature range, via geothermal cooling. The transfer assembly may also connect a holding facility, which may be dimensioned and structured to transfer mature marine life, possibly on an on-demand basis, to the harvesting facility.

A decentralized wastewater reuse design utilizing trickling filter (TF)-based aerobic bioreactors responds to the growing need for efficient energy usage per gallon of wastewater treated and/or pound of biological oxygen demand (BOD) removed from processed influent. A facility based on this design is able to adjust power consumption as needed due to external factors, such as utility rate scheduling, grid availability, and/or renewable power sources, without compromising effluent quality performance or increasing energy intensity. The facility improves on past TF applications by overcoming physical hydraulic constraints and expanding the capacity for both aerobic nitrification and anaerobic denitrification throughout the system. This design reduces grid dependency and overall power utilization per gallon of wastewater treated and/or per pound of BOD removal in alignment with climate-oriented policies that are expected to further exert pressure on states and municipalities to shift to carbon-free energy sources supplying all of their water/wastewater facility operations.

Provided is a purification method for water to be used for culture of an aquatic organism, wherein the method includes a nitrification step of oxidizing ammonia to nitric acid using a nitrifying bacterium adhering to a base material containing an alkaline earth metal, and a denitrification step of reducing nitric acid to nitrogen using a denitrifying bacterium adhering to a base material containing a biodegradable resin which has a structural unit derived from dicarboxylic acid.