The present disclosure provides novel polypeptides with 3-buten-2-ol dehydratase activity, polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene, and crystal structure data for one of such polypeptides. Methods of making and using the polypeptides and their related crystal structure data are also provided.

A hydrocarbon production method for producing hydrocarbons from a hydrocarbon mixture includes: a first extractive distillation step of performing extractive distillation of an extractive distillation target to obtain a fraction (A) in which isoprene and piperylene are enriched and a fraction (B) in which a linear hydrocarbon and a branched hydrocarbon are enriched; a first distillation step of obtaining a fraction (C) in which isoprene is enriched and a fraction (D) in which piperylene is enriched from the fraction (A); a dehydrogenation step of performing dehydrogenation or oxidative dehydrogenation of either or both of the linear hydrocarbon and the branched hydrocarbon contained in the fraction (B) to obtain a dehydrogenated product; and a recovery step of supplying the dehydrogenated product to an extractive distillation column or a distillation column and obtaining isoprene and/or piperylene from the dehydrogenated product.

A hydrocarbon production method for producing hydrocarbons from a hydrocarbon mixture includes: a first extractive distillation step of performing extractive distillation of an extractive distillation target to obtain a fraction (A) in which isoprene and piperylene are enriched and a fraction (B) in which a linear hydrocarbon and a branched hydrocarbon are enriched; a first distillation step of obtaining a fraction (C) in which isoprene is enriched and a fraction (D) in which piperylene is enriched from the fraction (A); a dehydrogenation step of performing dehydrogenation or oxidative dehydrogenation of either or both of the linear hydrocarbon and the branched hydrocarbon contained in the fraction (B) to obtain a dehydrogenated product; and a recovery step of supplying the dehydrogenated product to an extractive distillation column or a distillation column and obtaining isoprene and/or piperylene from the dehydrogenated product.

A zeolite membrane composite includes a porous support and a zeolite membrane formed on at least one surface of the porous support. The zeolite membrane of the zeolite membrane composite is formed of an X-MOR-type zeolite, where X includes at least one type of transition metal ion.

A zeolite membrane composite includes a porous support and a zeolite membrane formed on at least one surface of the porous support. The zeolite membrane of the zeolite membrane composite is formed of an X-MOR-type zeolite, where X includes at least one type of transition metal ion.

The present disclosure provides novel polypeptides with 3-buten-2-ol dehydratase activity, polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene, and crystal structure data for one of such polypeptides. Methods of making and using the polypeptides and their related crystal structure data are also provided.

The present disclosure provides novel polypeptides with 3-buten-2-ol dehydratase activity, polypeptides with catalytic activity in the conversion of 3-methyl-3-buten-2-ol to isoprene, and crystal structure data for one of such polypeptides. Methods of making and using the polypeptides and their related crystal structure data are also provided.

Disclosed herein is a process for separation and purification of a fermentation gas product from a fermenter off-gas containing a fermentation product, volatile impurities, bio-product impurities and water, based on a membrane system. In one preferred embodiment of the present invention, the process of separating and purifying a fermentation product from a fermenter off-gas, containing the fermentation product, volatile impurities, bio-byproduct impurities and water, comprises the steps of: (a) compressing the fermenter off-gas and feeding it into a membrane module, and (b) selectively permeating the volatile impurities, bio-byproduct impurities and water through the membrane, resulting in a product rich retentate stream. The fermentation gas product is separated from the fermenter off-gas in a single membrane stage to produce a purified retentate stream. In accordance with this embodiment of the invention, the fermenter off-gas (2), comprising the fermentation gas product, volatile impurities, bio-byproduct impurities and water, passes through a blower (201) that transports it to a gas holder (202), in order to smooth the process fluctuations. Stream (11) exits the gas holder (202) and is compressed in a multi-stage compressor (203), from which bio-byproduct and water are removed through stream (4). The compressed stream (12) enters the membrane module (204), which permeates the volatile impurities, disposing them in stream (13). The process may be still conducted in a 2-stage membrane system in accordance with the present invention.

Disclosed herein is a process for separation and purification of a fermentation gas product from a fermenter off-gas containing a fermentation product, volatile impurities, bio-product impurities and water, based on a membrane system. In one preferred embodiment of the present invention, the process of separating and purifying a fermentation product from a fermenter off-gas, containing the fermentation product, volatile impurities, bio-byproduct impurities and water, comprises the steps of: (a) compressing the fermenter off-gas and feeding it into a membrane module, and (b) selectively permeating the volatile impurities, bio-byproduct impurities and water through the membrane, resulting in a product rich retentate stream. The fermentation gas product is separated from the fermenter off-gas in a single membrane stage to produce a purified retentate stream. In accordance with this embodiment of the invention, the fermenter off-gas (2), comprising the fermentation gas product, volatile impurities, bio-byproduct impurities and water, passes through a blower (201) that transports it to a gas holder (202), in order to smooth the process fluctuations. Stream (11) exits the gas holder (202) and is compressed in a multi-stage compressor (203), from which bio-byproduct and water are removed through stream (4). The compressed stream (12) enters the membrane module (204), which permeates the volatile impurities, disposing them in stream (13). The process may be still conducted in a 2-stage membrane system in accordance with the present invention.

This application describes methods, including non-naturally occurring methods, for biosynthesizing 3-hydroxy-3-methylglutaryl-coA and intermediates thereof, as well as non-naturally occurring hosts for producing 3-hydroxy-3-methylglutaryl-coA. This application also describes methods, including non-naturally occurring methods, for biosynthesizing isoprene and intermediates thereof, as well as non-naturally occurring hosts for producing isoprene.