The present disclosure provides a method for photocleavage of an amide bond, the method has mild reaction conditions and can realize the cleavage of amide bonds by using light. The method comprises the following steps: subjecting 2,4-dinitrofluorobenzene to a reaction with an amino group of a substance represented by structural formula I with an α-amino acid at the end to produce a compound 1 represented by structural formula II; then under light irradiation, subjecting the compound 1 to a cleavage reaction of amide bond;

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Wherein, R1 is the side chain group of α-amino acid; R2 is: aryl, aliphatic hydrocarbon, —CH(R)—COOH or polypeptide.

The present disclosure provides methods of preparing a compound of Formula (1): wherein R.sup.1, R.sup.2, and R.sup.3 are as defined as set forth in the application. In one embodiment, a compound of Formula (1) is prepared in >95% chemical purity and >95% enantiomeric excess.

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The present disclosure provides methods of preparing a compound of Formula (1): wherein R.sup.1, R.sup.2, and R.sup.3 are as defined as set forth in the application. In one embodiment, a compound of Formula (1) is prepared in >95% chemical purity and >95% enantiomeric excess.

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The present disclosure is directed synthetic methods for the preparation of 4-valyloxybutyric acid. The synthetic methods described herein employ a diverse array of protecting group strategies and reaction conditions. Additionally, the present disclosure is directed to compounds useful as synthetic intermediates in the preparation of 4-valyloxybutyric acid.

The present disclosure is directed synthetic methods for the preparation of 4-valyloxybutyric acid. The synthetic methods described herein employ a diverse array of protecting group strategies and reaction conditions. Additionally, the present disclosure is directed to compounds useful as synthetic intermediates in the preparation of 4-valyloxybutyric acid.

The present disclosure is directed synthetic methods for the preparation of 4-valyloxybutyric acid. The synthetic methods described herein employ a diverse array of protecting group strategies and reaction conditions. Additionally, the present disclosure is directed to compounds useful as synthetic intermediates in the preparation of 4-valyloxybutyric acid.

A method is provided for deconstructing macromolecules (MM) into lower molecular weight (MW) fragments in high yield by promoting first desirable reactions (Reactions1) that result in chemolytic scission of bonds in the backbone, chain, matrix, or network that defines the MM and obtain a first product mixture (Product1). The method includes conveying the prepared feedstock in a flowpath toward a reactor while adding a first agent of a first type (A1T1) suitable for promoting Reactions1, and a second agent (A2) suitable for promoting Reactions1 to obtain a first reaction mixture which is heated under controlled pressure.

A method is provided for deconstructing macromolecules (MM) into lower molecular weight (MW) fragments in high yield by promoting first desirable reactions (Reactions1) that result in chemolytic scission of bonds in the backbone, chain, matrix, or network that defines the MM and obtain a first product mixture (Product1). The method includes conveying the prepared feedstock in a flowpath toward a reactor while adding a first agent of a first type (A1T1) suitable for promoting Reactions1, and a second agent (A2) suitable for promoting Reactions1 to obtain a first reaction mixture which is heated under controlled pressure.

The invention provides a process for preparing a TCA cycle intermediate conjugated to an amino acid having the structure of a compound of Formula (X):

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The invention provides a process for preparing a TCA cycle intermediate conjugated to an amino acid having the structure of a compound of Formula (X):

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