1,3-Dipole-functional compounds (e.g., azide functional compounds) can be reacted with certain alkynes in a cyclization reaction to form heterocyclic compounds. Useful alkynes (e.g., strained, cyclic alkynes) and methods of making such alkynes are also disclosed. The reaction of 1,3-dipole-functional compounds with alkynes can be used for a wide variety of applications including the immobilization of biomolecules on a substrate.

A 2,6-dibenzylidenecyclohexanone oxime compound and a preparation method and use thereof are provided. A structural fragment with anti-inflammatory activity is introduced into the 2,6-dibenzylidenecyclohexanone oxime compound, and the prepared compound has stable physicochemical properties. The 2,6-dibenzylidenecyclohexanone oxime compound has an excellent inhibitory effect on inflammatory factors and better in-vivo anti-inflammatory activity, and especially has a protective effect on hepatitis and fatty liver caused by excessive expression and release of TNF- and/or IL-6, as well as liver inflammation and liver lipid metabolism.

A 2,6-dibenzylidenecyclohexanone oxime compound and a preparation method and use thereof are provided. A structural fragment with anti-inflammatory activity is introduced into the 2,6-dibenzylidenecyclohexanone oxime compound, and the prepared compound has stable physicochemical properties. The 2,6-dibenzylidenecyclohexanone oxime compound has an excellent inhibitory effect on inflammatory factors and better in-vivo anti-inflammatory activity, and especially has a protective effect on hepatitis and fatty liver caused by excessive expression and release of TNF- and/or IL-6, as well as liver inflammation and liver lipid metabolism.