Disclosed is a method for production of oxygenated diterpenoid compounds, such as triptophenolide, triptonide and triptolide, by inserting genes encoding particular cytochrome P450 enzymes and expressing the genes in selected host cells for synthesis of the compounds. Further disclosed are particular cytochrome P450 enzymes suitable for this synthesis.

Disclosed is a method for production of oxygenated diterpenoid compounds, such as triptophenolide, triptonide and triptolide, by inserting genes encoding particular cytochrome P450 enzymes and expressing the genes in selected host cells for synthesis of the compounds. Further disclosed are particular cytochrome P450 enzymes suitable for this synthesis.

Isoindoline sigma-2 receptor antagonist compounds, pharmaceutical compositions comprising such compounds, and methods for inhibiting Abeta-associated synapse loss or synaptic dysfunction in neuronal cells, modulating an Abeta-associated membrane trafficking change in neuronal cells, and treating cognitive decline associated with Abeta pathology are provided.

Isoindoline sigma-2 receptor antagonist compounds, pharmaceutical compositions comprising such compounds, and methods for inhibiting Abeta-associated synapse loss or synaptic dysfunction in neuronal cells, modulating an Abeta-associated membrane trafficking change in neuronal cells, and treating cognitive decline associated with Abeta pathology are provided.