Provided herein are, inter alia, biological manufacturing and downstream purification processes.

The present invention provides a protein separation micro el having a first plate having a first opening; a second plate; a gel substrate formed between the first plate and the second plate; and a plurality of wells positioned at a first non-zero distance from a first edge of the gel and at a second non-zero distance from a second edge of the gel; wherein the first and second edge of the gel are opposite edges of the gel.

The present invention provides a protein separation micro el having a first plate having a first opening; a second plate; a gel substrate formed between the first plate and the second plate; and a plurality of wells positioned at a first non-zero distance from a first edge of the gel and at a second non-zero distance from a second edge of the gel; wherein the first and second edge of the gel are opposite edges of the gel.

Provided herein are, inter alia, biological manufacturing and downstream purification processes.

Provided herein are, inter alia, biological manufacturing and downstream purification processes.

Provided herein are, inter alia, biological manufacturing and downstream purification processes.

Provided herein are, inter alia, biological manufacturing and downstream purification processes.

Provided herein are, inter alia, biological manufacturing and downstream purification processes.

Provided herein are, inter alia, biological manufacturing and downstream purification processes.

Some embodiments described herein relate to systems and methods operable to combine immunoassay and Total Protein techniques in a single sample run. Some embodiments described herein allow for multiple sequential immunoassays to be performed in the same microfluidic device. Some embodiments described herein relate to stripping reagents operable to remove primary antibodies associated with immunoassays. Such stripping reagents can allow for additional immunoassays and/or Total Protein assays to be performed on the same sample.