A method of producing protein products including alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins from plasma includes steps of: (1) adding a salt to the blood product to produce a first intermediate, wherein the salt comprises between 11-13 wt % of the first intermediate; (2) separating the first intermediate to produce a first supernatant and a first paste; (3) adding a salt to the first intermediate to produce a second intermediate, wherein the salt comprises between 21-23 wt % of the second intermediate; (4) separating the second intermediate to produce a second supernatant and a second paste; (5) separating a third intermediate from the second supernatant by affinity chromatography; and (6) separating the third intermediate by ion exchange chromatography to produce an eluate containing the protein product. Advantageously, the inventive methods are simple and produce alpha-1-proteinase inhibitor, gamma globulin, albumin, and other proteins in high yields.

A method for producing a mammalian brain protein hydrolysate composition, by providing a mammalian brain protein hydrolysate, adding Zn ions to the mammalian brain protein hydrolysate to form a complex with the Zn ions and the mammalian brain protein hydrolysate, and obtaining the complex with the Zn ions and the mammalian brain protein hydrolysate in a mammalian brain protein hydrolysate composition. A composition with a mammalian brain protein hydrolysate. A nutritional supplement with a mammalian brain protein hydrolysate. A pharmaceutical composition with a mammalian brain protein hydrolysate.

A method for producing a mammalian brain protein hydrolysate composition, by providing a mammalian brain protein hydrolysate, adding Zn ions to the mammalian brain protein hydrolysate to form a complex with the Zn ions and the mammalian brain protein hydrolysate, and obtaining the complex with the Zn ions and the mammalian brain protein hydrolysate in a mammalian brain protein hydrolysate composition. A composition with a mammalian brain protein hydrolysate. A nutritional supplement with a mammalian brain protein hydrolysate. A pharmaceutical composition with a mammalian brain protein hydrolysate.

A method for separating soluble proteins from chlorophyll using a water-soluble silicate. Various purified products and intermediates are provided.

A method for separating soluble proteins from chlorophyll using a water-soluble silicate. Various purified products and intermediates are provided.

Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.

Methods of purifying proteins expressed in non-mammalian expression systems in a non-native soluble form directly from cell lysate are disclosed. Methods of purifying proteins expressed in non-mammalian expression systems in a non-native limited solubility form directly from a refold solution are also disclosed. Resin regeneration methods are also provided.

This invention relates to the methods of preparation of a growth factor concentrate derived from platelet lysate and modified to be devoid of fibrinogen. Moreover, the present invention concerns therapeutic applications of said growth factor concentrate to enhance mitogenic, osteogenic, chondrogenic and angiogenic abilities. The disclosed preparation can be used immediately, or stored frozen until use, or lyophilized, or mixed with pharmaceutical formulation, such as biodegradable polymer or other delivery vehicles, to make products.

This invention relates to the methods of preparation of a growth factor concentrate derived from platelet lysate and modified to be devoid of fibrinogen. Moreover, the present invention concerns therapeutic applications of said growth factor concentrate to enhance mitogenic, osteogenic, chondrogenic and angiogenic abilities. The disclosed preparation can be used immediately, or stored frozen until use, or lyophilized, or mixed with pharmaceutical formulation, such as biodegradable polymer or other delivery vehicles, to make products.

Processes for preparing and purifying protein from plant material, and compositions and uses comprising the same, are provided.