The present application relates to a replicable RNA molecule and the use thereof. The replicable RNA molecule comprises, from the 5 end to the 3 end, a 5 cap, a 5 UTR, an open reading frame encoding an RNA replicase, a promoter, a sequence of interest, a 3 UTR and a poly(A) tail, wherein the RNA replicase is capable of amplifying the replicable RNA molecule and is capable of amplifying an RNA molecule containing the sequence of interest and 3 UTR, wherein the RNA replicase is a nonstructural protein or a functional variant thereof derived from Mosso das Pedras virus (MDPV), Everglades virus (EVEV), Rio Negro virus (RNV), Mucambo virus (MUCV), Highlands J virus (HJV), Pixuna virus (PIXV), Trocara virus (TROV), Cabassou virus (CABV), Tonate virus (TONV), Bebaru virus (BEBV), Fort Morgan virus (FMV), Getah virus (GETV) or Ndumu virus (NDUV).

The present invention relates to stabilized human parainfluenza virus 1 (HPIV1) F protein, and to nucleic acid sequences encoding such proteins, as well as to uses of said proteins and nucleic acid sequences.

The present invention relates to stabilized human parainfluenza virus 1 (HPIV1) F protein, and to nucleic acid sequences encoding such proteins, as well as to uses of said proteins and nucleic acid sequences.