Provided is a method for intracellularly producing a large amount of a Cry protein in a Bacillus bacterium. A method for producing a Cry protein or a culture product comprising the Cry protein, comprising transforming a Bacillus bacterium with an expression plasmid incorporating a gene encoding the Cry protein operably linked to a regulatory region comprising a σA-dependent promoter or a σH-dependent promoter, and culturing the transformed cell, wherein the expression plasmid comprises a polynucleotide encoding a replication protein consisting of the amino acid sequence set forth in SEQ ID NO: 9 or a protein having an identity of 80% or more or more with the amino acid sequence of the replication protein and involved in replication initiation.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

The invention provides nucleic acids, and variants and fragments thereof, obtained from strains of Bacillus thuringiensis encoding polypeptides having pesticidal activity against insect pests, including Lepidoptera and Coleoptera. Particular embodiments of the invention provide isolated nucleic acids encoding pesticidal proteins, pesticidal compositions, DNA constructs, and transformed microorganisms and plants comprising a nucleic acid of the embodiments. These compositions find use in methods for controlling pests, especially plant pests.

Provided are novel recombinant proteins that are capable of self-crystallization and exhibit desirable physical properties such as enhanced cellular uptake or and endoso-mal escape. Polynucleotides encoding the recombinant proteins as well as methods of making and using such proteins are also described.

Provided are novel recombinant proteins that are capable of self-crystallization and exhibit desirable physical properties such as enhanced cellular uptake or and endoso-mal escape. Polynucleotides encoding the recombinant proteins as well as methods of making and using such proteins are also described.

Compositions and methods for conferring pesticidal activity to bacteria, plants, plant cells, tissues and seeds are provided. Compositions comprising a coding sequence for a toxin polypeptide are provided. The coding sequences can be used in DNA constructs or expression cassettes for transformation and expression in plants and bacteria. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated toxin nucleic acid molecules are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed, and antibodies specifically binding to those amino acid sequences. In particular, the present invention provides for isolated nucleic acid molecules comprising nucleotide sequences encoding the amino acid sequence shown in any of SEQ ID NO: 22 to 42, or the nucleotide sequence set forth in any of SEQ ID NO: 1 to 21, as well as variants and fragments thereof.

The present invention relates to a novel transgenic maize event expressing the truncated or modified Cry1Da insecticidal protein designated as Event ME240913. The invention relates to nucleic acids that are unique to Event ME240913. Primers, amplicon, methods and kits for detecting the presence of Event ME240913 are also defined. The invention further relates to maize plants containing said event, uses thereof, methods and compositions for controlling lepidopteran insect pests. The invention describes a maize event that has shown a high level of plant protection against feeding damage caused by Lepidoptera, including S. frugirperda, as well as Cry1F resistant insects. The event of the invention was shown to be highly toxic to S. frugirperda.

The present invention relates to a novel transgenic maize event expressing the truncated or modified Cry1Da insecticidal protein designated as Event ME240913. The invention relates to nucleic acids that are unique to Event ME240913. Primers, amplicon, methods and kits for detecting the presence of Event ME240913 are also defined. The invention further relates to maize plants containing said event, uses thereof, methods and compositions for controlling lepidopteran insect pests. The invention describes a maize event that has shown a high level of plant protection against feeding damage caused by Lepidoptera, including S. frugirperda, as well as Cry1F resistant insects. The event of the invention was shown to be highly toxic to S. frugirperda.

The invention provides nucleic acids, polypeptides, transgenic plants, compositions and methods for conferring pesticidal activity (e.g., insecticidal activity) to bacteria, plants, plant cells, tissues and seeds. Nucleic acids encoding the insecticidal proteins can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. The recombinant organisms or compositions containing the recombinant organisms or insecticidal proteins or in combination with an appropriate agricultural carrier can be used to control an insect pest in various environments.

The invention provides nucleic acids, polypeptides, transgenic plants, compositions and methods for conferring pesticidal activity (e.g., insecticidal activity) to bacteria, plants, plant cells, tissues and seeds. Nucleic acids encoding the insecticidal proteins can be used to transform prokaryotic and eukaryotic organisms to express the insecticidal proteins. The recombinant organisms or compositions containing the recombinant organisms or insecticidal proteins or in combination with an appropriate agricultural carrier can be used to control an insect pest in various environments.