Components for enabling immunodection of MT-45 are described including immunogens, antibodies derived from the immunogens, immunoassay methods, detecting agents and kits.

Components for enabling immunodection of MT-45 are described including immunogens, antibodies derived from the immunogens, immunoassay methods, detecting agents and kits.

Methods of treating individuals with a glucose metabolism disorder and/or a body weight disorder, and compositions associated therewith, are provided.

Methods of treating individuals with a glucose metabolism disorder and/or a body weight disorder, and compositions associated therewith, are provided.

Methods of treating individuals with a glucose metabolism disorder and/or a body weight disorder, and compositions associated therewith, are provided.

Methods of treating individuals with a glucose metabolism disorder and/or a body weight disorder, and compositions associated therewith, are provided.

An object of the present invention is to suppress the variation of the elution position of a compound having a charged portion by a preservation liquid, in the purification of the compound, without carrying out the substitution step of the preservation liquid attached to the adsorbent used for the purification and the keeping step. A method for purifying a compound having a charged portion, the method comprising the steps of: preparing a composition containing a compound having a charged portion; preparing a buffer solution comprising a buffering agent and an alcohol, the buffer containing a calcium phosphate compound at least partially, having a buffer capacity in a range of pH 6.0 to pH 8.0, and being soluble in a polar solvent and insoluble in a non-polar solvent; preserving an adsorbent in the buffer solution; adsorbing the compound on the adsorbent by bringing the composition into contact with the adsorbent preserved in the buffer solution; and separating the compound from the adsorbent by gradient elution.

Disclosed are a reagent kit for removing a bacterial endotoxin in a biological product, a method for using the reagent kit for removing the bacterial endotoxin in the biological product, a method for preparing an endotoxin-free biological product, and the endotoxin-free biological product thus produced. The reagent kit of the present invention comprises an anionic surfactant and a potassium salt. When in use, the anionic surfactant is fully bonded with the endotoxin in the biological product to form a conjugate, then the potassium salt is added to precipitate the conjugate, the precipitate is removed by filtration to produce a biological product solution with the endotoxin removed, and then the biological product is separated from the biological product solution to complete the process.

Disclosed are a reagent kit for removing a bacterial endotoxin in a biological product, a method for using the reagent kit for removing the bacterial endotoxin in the biological product, a method for preparing an endotoxin-free biological product, and the endotoxin-free biological product thus produced. The reagent kit of the present invention comprises an anionic surfactant and a potassium salt. When in use, the anionic surfactant is fully bonded with the endotoxin in the biological product to form a conjugate, then the potassium salt is added to precipitate the conjugate, the precipitate is removed by filtration to produce a biological product solution with the endotoxin removed, and then the biological product is separated from the biological product solution to complete the process.

The present invention relates to Protoxin-II variants, polynucleotides encoding them, and methods of making and using the foregoing.